Mouse Anti-NANOG Recombinant Antibody (
8A1D11) (V2LY-0725-LY515)

Name: Mouse Anti-NANOG Recombinant Antibody (8A1D11)
SKU: V2LY-0725-LY515
Rating: 5 (1 reviews)

See all NANOG antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in WB

Western Blot
Positive WB detected in: Rat brain tissue, Mouse brain tissue
All lanes: NANOG antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 42 kDa

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate
All lanes: NANOG antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 40 kDa

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in ICC

Immunocytochemistry analysis of V2LY-0725-LY515 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in ICC

Immunocytochemistry analysis of V2LY-0725-LY515 diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in IF

Immunofluorescence staining of Hela cells with V2LY-0725-LY515 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in IF

Immunofluorescence staining of Ntera-2 cells with V2LY-0725-LY515 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY515 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in FC

Overlay histogram showing MCF-7 cells stained with V2LY-0725-LY515 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (V2LY-0725-LY515) in FC

Overlay histogram showing Ntera-2 cells stained with V2LY-0725-LY515 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8A1D11

Application

ELISA, WB, ICC, IF, FC

Immunogen

Recombinant Human Homeobox protein NANOG protein (aa 1-305).

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:50-1:500
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Nanog Homeobox

Entrez Gene ID

79923

UniProt ID

Q9H9S0

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768).

Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Biological Process

Cell differentiation Source: HGNC-UCL
Endodermal cell fate specification Source: MGI
Positive regulation of stem cell proliferation Source: HGNC-UCL
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of cell differentiation Source: HGNC-UCL
Regulation of gene expression Source: UniProtKB
Regulation of transcription, DNA-templated Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Somatic stem cell population maintenance Source: UniProtKB
Stem cell population maintenance Source: BHF-UCL

Cellular Location

Nucleus

Kuznetsova, K., Chabot, N. M., Ugolini, M., Wu, E., Lalit, M., Oda, H., ... & Vastenhouw, N. L. (2023). Nanog organizes transcription bodies. Current Biology, 33(1), 164-173.

Vasefifar, P., Motafakkerazad, R., Maleki, L. A., Najafi, S., Ghrobaninezhad, F., Najafzadeh, B., ... & Baradaran, B. (2022). Nanog, as a key cancer stem cell marker in tumor progression. Gene, 827, 146448.

Yoon, C., Lu, J., Yi, B. C., Chang, K. K., Simon, M. C., Ryeom, S., & Yoon, S. S. (2021). PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas. Oncogenesis, 10(1), 12.

Najafzadeh, B., Asadzadeh, Z., Motafakker Azad, R., Mokhtarzadeh, A., Baghbanzadeh, A., Alemohammad, H., ... & Baradaran, B. (2021). The oncogenic potential of NANOG: An important cancer induction mediator. Journal of cellular physiology, 236(4), 2443-2458.

Springer, C., Zakhartchenko, V., Wolf, E., & Simmet, K. (2021). Hypoblast formation in bovine embryos does not depend on NANOG. Cells, 10(9), 2232.

Fatma, H., Siddique, H. R., & Maurya, S. K. (2021). The multiple faces of NANOG in cancer: a therapeutic target to chemosensitize therapy-resistant cancers. Epigenomics, 13(23), 1885-1900.

Grubelnik, G., Boštjančič, E., Pavlič, A., Kos, M., & Zidar, N. (2020). NANOG expression in human development and cancerogenesis. Experimental Biology and Medicine, 245(5), 456-464.

Grubelnik, G., Boštjančič, E., Grošelj, A., & Zidar, N. (2020). Expression of NANOG and its regulation in oral squamous cell carcinoma. BioMed Research International, 2020.

Mahalaxmi, I., Devi, S. M., Kaavya, J., Arul, N., Balachandar, V., & Santhy, K. S. (2019). New insight into NANOG: A novel therapeutic target for ovarian cancer (OC). European journal of pharmacology, 852, 51-57.

Heurtier, V., Owens, N., Gonzalez, I., Mueller, F., Proux, C., Mornico, D., ... & Navarro, P. (2019). The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nature communications, 10(1), 1109.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-NANOG Recombinant Antibody (
8A1D11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-NANOG Recombinant Antibody (8A1D11) (CAT#: CBMAB-R4324-CN)

Related Products

Mouse Anti-NANOG Recombinant Antibody (2C11) (CAT#: CBMAB-N1109-WJ)

Mouse Anti-NANOG Recombinant Antibody (CBT936) (CAT#: V2LY-0625-LY3169)

Mouse Anti-NANOG Recombinant Antibody (F-8) (CAT#: CBMAB-N1114-WJ)

Mouse Anti-NANOG Recombinant Antibody (10E42) (CAT#: CBMAB-N1106-WJ)

Mouse Anti-NANOG Recombinant Antibody (CBWJN-0299) (CAT#: CBMAB-N0115-WJ)

Mouse Anti-NANOG Recombinant Antibody (NNG-811) (CAT#: CBMAB-N1118-WJ)

Mouse Anti-NANOG Recombinant Antibody (P5A10AT) (CAT#: CBMAB-N1119-WJ)

Mouse Anti-NANOG Recombinant Antibody (PR-2E11) (CAT#: CBMAB-N1120-WJ)

Rabbit Anti-NANOG Recombinant Antibody (D1G10) (CAT#: CBMAB-N1112-WJ)

Mouse Anti-NANOG Recombinant Antibody (CBWJN-1165) (CAT#: CBMAB-N1102-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry