Mouse Anti-NANOS1 Recombinant Antibody (N1) (CBMAB-N1124-WJ)

Name: Mouse Anti-NANOS1 Recombinant Antibody (N1)
SKU: CBMAB-N1124-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, WB

Specificity

Goat, Human, Pig, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nanos homolog 1 (Drosophila)

Introduction

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Entrez Gene ID

Human	340719
Pig	100157783
Rat	365494
Goat	108634026

UniProt ID

Human	Q8WY41
Pig	A0A286ZUB3
Rat	D4A1F8
Goat	A0A452DQF8

Alternative Names

Nanos C2HC-Type Zinc Finger 1; EC_Rep1a; NOS-1; NOS1; Nanos Homolog 1 (Drosophila); Nanos Homolog 1; ZC2HC12A; SPGF12;

Function

May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.

Biological Process

Cell migration Source: UniProtKB
Cerebellar neuron development Source: Ensembl
Epithelial cell migration Source: UniProtKB
Negative regulation of translation Source: BHF-UCL
Oogenesis Source: GO_Central
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: BHF-UCL
Posttranscriptional regulation of gene expression Source: MGI
Regulation of cell growth Source: Ensembl
Tissue homeostasis Source: Ensembl

Cellular Location

Cytoplasm
Other locations
perinuclear region
Note: Colocalizes with SNAPIN and PUM2 in the perinuclear region of germ cells.

Involvement in disease

Spermatogenic failure 12 (SPGF12):
An infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. Non-obstructive azoospermia, oligozoospermia and oligo-astheno-teratozoospermia are features observed in SPGF12 patients.

Maschi, D., Fernández-Alvarez, A. J., & Boccaccio, G. L. (2023). The RNA-binding protein NANOS1 controls hippocampal synaptogenesis. PloS one, 18(4), e0284589.

Ilaslan, E., Kwiatkowska, K., Smialek, M. J., Sajek, M. P., Lemanska, Z., Alla, M., ... & Kusz-Zamelczyk, K. (2022). Distinct roles of NANOS1 and NANOS3 in the cell cycle and NANOS3-PUM1-FOXM1 axis to control G2/M phase in a human primordial germ cell model. International Journal of Molecular Sciences, 23(12), 6592.

Liu, L., Liu, T., Wu, S., Li, Y., Wei, H., Zhang, L., ... & Zhang, L. (2022). Discovery of Nanos1 and Nanos2/3 as germ cell markers during scallop gonadal development. Marine Biotechnology, 24(2), 408-416.

Na, H., Park, J., Jeon, H., Jin, S., & Choe, C. P. (2021). Pharyngeal endoderm expression of nanos1 is dispensable for craniofacial development. Gene Expression Patterns, 41, 119202.

Janecki, D. M., Ilaslan, E., Smialek, M. J., Sajek, M. P., Kotecki, M., Ginter-Matuszewska, B., ... & Kusz-Zamelczyk, K. (2020). Human NANOS1 represses apoptosis by downregulating pro-apoptotic genes in the male germ cell line. International Journal of Molecular Sciences, 21(8), 3009.

Babich, B., Roba, G., Safura, S. S., Callahan, K., & Freeman, E. A. (2019). Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR. American Journal of Undergraduate Research, 16(1), 15.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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