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Mouse Anti-NAPEPLD Recombinant Antibody (1H4) (CBMAB-N1160-WJ)

This product is a Mouse antibody that recognizes NAPEPLD. The antibody 1H4 can be used for immunoassay techniques such as: WB, IF .
See all NAPEPLD antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1H4
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.94 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-Acyl Phosphatidylethanolamine Phospholipase D
Introduction
NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008]
Entrez Gene ID
222236
UniProt ID
Q6IQ20
Alternative Names
N-Acyl Phosphatidylethanolamine Phospholipase D; NAPE-Hydrolyzing Phospholipase D; NAPE-PLD; C7orf18; Chromosome 7 Open Reading Frame 18, N-Acyl-Phosphatidylethanolamine-Hydrolyzing Phospholipase D; N-Acyl-Phosphatidylethanolamine-Hydrolyzing Phospholipase D; EC 3.1.4.54; EC 3.1.4; FMP30;
Function
D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid (PubMed:14634025, PubMed:16527816, PubMed:27571266, PubMed:25684574).

Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain (By similarity).

May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation (By similarity).

As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs (By similarity).
Biological Process
Host-mediated regulation of intestinal microbiota composition Source: UniProtKB
N-acylethanolamine metabolic process Source: GO_Central
N-acylphosphatidylethanolamine metabolic process Source: UniProtKB
Phospholipid catabolic process Source: UniProtKB-KW
Positive regulation of brown fat cell differentiation Source: UniProtKB
Positive regulation of inflammatory response Source: UniProtKB
Retinoid metabolic process Source: Reactome
Temperature homeostasis Source: UniProtKB
Cellular Location
Nucleus
Nucleus envelope
nucleoplasm
Golgi apparatus
Golgi apparatus membrane
Endosome
Early endosome membrane
Note: Localized in the proximity of the cellular membranes likely through interaction with membrane phospholipids.

Zarrow, J. E., Alli-Oluwafuyi, A. M., Youwakim, C. M., Kim, K., Jenkins, A. N., Suero, I. C., ... & Davies, S. S. (2023). Small molecule activation of NAPE-PLD enhances efferocytosis by macrophages. ACS chemical biology, 18(8), 1891-1904.

Tevosian, M., Todorov, H., Lomazzo, E., Bindila, L., Ueda, N., Bassetti, D., ... & Lutz, B. (2023). NAPE-PLD deletion in stress-TRAPed neurons results in an anxiogenic phenotype. Translational Psychiatry, 13(1), 152.

Chen, I., Murdaugh, L. B., Miliano, C., Dong, Y., Gregus, A. M., & Buczynski, M. W. (2023). NAPE-PLD regulates specific baseline affective behaviors but is dispensable for inflammatory hyperalgesia. Neurobiology of Pain, 14, 100135.

Zarrow, J. E., Tian, J., Dutter, B., Kim, K., Doran, A. C., Sulikowski, G. A., & Davies, S. S. (2022). Selective measurement of NAPE-PLD activity via a PLA1/2-resistant fluorogenic N-acyl-phosphatidylethanolamine analog. Journal of lipid research, 63(1).

Mock, E. D., Driever, W. P., & van der Stelt, M. (2022). Fluorescence-based NAPE-PLD activity assay. In Endocannabinoid Signaling: Methods and Protocols (pp. 233-240). New York, NY: Springer US.

Li, J. A., Rong, Y., Mao, W., Zhang, L., Kuang, T., & Lou, W. (2022). Gene expression profiling reveals the genomic changes caused by MLN4924 and the sensitizing effects of NAPEPLD knockdown in pancreatic cancer. Cell cycle, 21(2), 152-171.

Lefort, C., Roumain, M., Van Hul, M., Rastelli, M., Manco, R., Leclercq, I., ... & Cani, P. D. (2020). Hepatic NAPE-PLD is a key regulator of liver lipid metabolism. Cells, 9(5), 1247.

Mock, E. D., Mustafa, M., Gunduz-Cinar, O., Cinar, R., Petrie, G. N., Kantae, V., ... & van der Stelt, M. (2020). Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice. Nature chemical biology, 16(6), 667-675.

Rastelli, M., Van Hul, M., Terrasi, R., Lefort, C., Régnier, M., Beiroa, D., ... & Cani, P. D. (2020). Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis. American Journal of Physiology-Endocrinology and Metabolism, 319(3), E647-E657.

Igarashi, M., Watanabe, K., Tsuduki, T., Kimura, I., & Kubota, N. (2019). NAPE‐PLD controls OEA synthesis and fat absorption by regulating lipoprotein synthesis in an in vitro model of intestinal epithelial cells. The FASEB Journal, 33(3), 3167-3179.

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For research use only. Not intended for any clinical use.

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