Mouse Anti-NAPEPLD Recombinant Antibody (5F3) (CBMAB-N1163-WJ)

Name: Mouse Anti-NAPEPLD Recombinant Antibody (5F3)
SKU: CBMAB-N1163-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5F3

Application

WB, IHC, FC

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.71 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

N-Acyl Phosphatidylethanolamine Phospholipase D

Introduction

NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008]

Entrez Gene ID

222236

UniProt ID

Q6IQ20

Alternative Names

N-Acyl Phosphatidylethanolamine Phospholipase D; NAPE-Hydrolyzing Phospholipase D; NAPE-PLD; C7orf18; Chromosome 7 Open Reading Frame 18, N-Acyl-Phosphatidylethanolamine-Hydrolyzing Phospholipase D; N-Acyl-Phosphatidylethanolamine-Hydrolyzing Phospholipase D; EC 3.1.4.54; EC 3.1.4; FMP30;

Function

D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid (PubMed:14634025, PubMed:16527816, PubMed:27571266, PubMed:25684574).

Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain (By similarity).

May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation (By similarity).

As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs (By similarity).

Biological Process

Host-mediated regulation of intestinal microbiota composition Source: UniProtKB
N-acylethanolamine metabolic process Source: GO_Central
N-acylphosphatidylethanolamine metabolic process Source: UniProtKB
Phospholipid catabolic process Source: UniProtKB-KW
Positive regulation of brown fat cell differentiation Source: UniProtKB
Positive regulation of inflammatory response Source: UniProtKB
Retinoid metabolic process Source: Reactome
Temperature homeostasis Source: UniProtKB

Cellular Location

Nucleus
Nucleus envelope
nucleoplasm
Golgi apparatus
Golgi apparatus membrane
Endosome
Early endosome membrane
Note: Localized in the proximity of the cellular membranes likely through interaction with membrane phospholipids.

Zarrow, J. E., Alli-Oluwafuyi, A. M., Youwakim, C. M., Kim, K., Jenkins, A. N., Suero, I. C., ... & Davies, S. S. (2023). Small molecule activation of NAPE-PLD enhances efferocytosis by macrophages. ACS chemical biology, 18(8), 1891-1904.

Tevosian, M., Todorov, H., Lomazzo, E., Bindila, L., Ueda, N., Bassetti, D., ... & Lutz, B. (2023). NAPE-PLD deletion in stress-TRAPed neurons results in an anxiogenic phenotype. Translational Psychiatry, 13(1), 152.

Chen, I., Murdaugh, L. B., Miliano, C., Dong, Y., Gregus, A. M., & Buczynski, M. W. (2023). NAPE-PLD regulates specific baseline affective behaviors but is dispensable for inflammatory hyperalgesia. Neurobiology of Pain, 14, 100135.

Zarrow, J. E., Tian, J., Dutter, B., Kim, K., Doran, A. C., Sulikowski, G. A., & Davies, S. S. (2022). Selective measurement of NAPE-PLD activity via a PLA1/2-resistant fluorogenic N-acyl-phosphatidylethanolamine analog. Journal of lipid research, 63(1).

Mock, E. D., Driever, W. P., & van der Stelt, M. (2022). Fluorescence-based NAPE-PLD activity assay. In Endocannabinoid Signaling: Methods and Protocols (pp. 233-240). New York, NY: Springer US.

Li, J. A., Rong, Y., Mao, W., Zhang, L., Kuang, T., & Lou, W. (2022). Gene expression profiling reveals the genomic changes caused by MLN4924 and the sensitizing effects of NAPEPLD knockdown in pancreatic cancer. Cell cycle, 21(2), 152-171.

Lefort, C., Roumain, M., Van Hul, M., Rastelli, M., Manco, R., Leclercq, I., ... & Cani, P. D. (2020). Hepatic NAPE-PLD is a key regulator of liver lipid metabolism. Cells, 9(5), 1247.

Mock, E. D., Mustafa, M., Gunduz-Cinar, O., Cinar, R., Petrie, G. N., Kantae, V., ... & van der Stelt, M. (2020). Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice. Nature chemical biology, 16(6), 667-675.

Rastelli, M., Van Hul, M., Terrasi, R., Lefort, C., Régnier, M., Beiroa, D., ... & Cani, P. D. (2020). Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis. American Journal of Physiology-Endocrinology and Metabolism, 319(3), E647-E657.

Igarashi, M., Watanabe, K., Tsuduki, T., Kimura, I., & Kubota, N. (2019). NAPE‐PLD controls OEA synthesis and fat absorption by regulating lipoprotein synthesis in an in vitro model of intestinal epithelial cells. The FASEB Journal, 33(3), 3167-3179.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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