Mouse Anti-NCOA3 Recombinant Antibody (CBXS-3650) (CBMAB-S0929-CQ)

Name: Mouse Anti-NCOA3 Recombinant Antibody (CBXS-3650)
SKU: CBMAB-S0929-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXS-3650

Application

FC, IF, WB

Immunogen

Recombinant human SRC-3 polypeptide fragment corresponding to amino acids 1-250

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.01 M HEPES, 0.15 M NaCl, 0.1 mg/mL BSA, 50% glycerol, pH 7.5

Preservative

0.2% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nuclear Receptor Coactivator 3

Introduction

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Entrez Gene ID

8202

UniProt ID

Q9Y6Q9

Alternative Names

Nuclear Receptor Coactivator 3; Thyroid Hormone Receptor Activator Molecule 1; Receptor-Associated Coactivator 3; Class E Basic Helix-Loop-Helix Protein 42; Steroid Receptor Coactivator Protein 3; Amplified In Breast Cancer 1 Protein; CBP-Interacting Protein; EC 2.3.1.48; BHLHe42; TRAM-1; AIB-1; SRC-3; AIB1;

Function

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

Biological Process

Cell dedifferentiation Source: UniProtKB
Cellular response to estradiol stimulus Source: BHF-UCL
Cellular response to hormone stimulus Source: GO_Central
Positive regulation of keratinocyte differentiation Source: UniProtKB
Positive regulation of stem cell population maintenance Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Receptor transactivation Source: UniProtKB
Regulation of stem cell division Source: UniProtKB

Cellular Location

Nucleus
Cytoplasm
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

PTM

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated
Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.

Kiliti, A. J., Sharif, G. M., Martin, M. B., Wellstein, A., & Riegel, A. T. (2023). AIB1/SRC-3/NCOA3 function in estrogen receptor alpha positive breast cancer. Frontiers in Endocrinology, 14, 1250218.

Li, X., Zhang, J., Wang, B., Chen, C., Zhang, E., Lv, Z., ... & Zhang, F. (2023). USP24-dependent stabilization of Runx2 recruits a p300/NCOA3 complex to transactivate ADAMTS genes and promote degeneration of intervertebral disc in chronic inflammation mice. Biology Direct, 18(1), 37.

Liu, D., Li, J., Hao, W., Lin, X., Xia, J., Zhu, J., ... & Yang, X. (2022). Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3. Frontiers in Veterinary Science, 9, 895190.

Li, Y., Liang, J., Dang, H., Zhang, R., Chen, P., & Shao, Y. (2022). NCOA3 is a critical oncogene in thyroid cancer via the modulation of major signaling pathways. Endocrine, 1-10.

Cui, Y., Fan, J., Shi, W., & Zhou, Z. (2022). Circ_0001667 knockdown blocks cancer progression and attenuates adriamycin resistance by depleting NCOA3 via releasing miR‐4458 in breast cancer. Drug Development Research, 83(1), 75-87.

de Semir, D., Bezrookove, V., Nosrati, M., Dar, A. A., Miller III, J. R., Leong, S. P., ... & Kashani-Sabet, M. (2021). Nuclear Receptor Coactivator NCOA3 Regulates UV Radiation–Induced DNA Damage and Melanoma Susceptibility. Cancer research, 81(11), 2956-2969.

Zheng, X., Jia, Y., Qiu, L., Zeng, X., Xu, L., Wei, M., ... & Han, J. (2020). A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator. Journal of Biological Chemistry, 295(6), 1474-1488.

Li, W., Yan, Y., Zheng, Z., Zhu, Q., Long, Q., Sui, S., ... & Li, L. (2020). Targeting the NCOA3-SP1-TERT axis for tumor growth in hepatocellular carcinoma. Cell death & disease, 11(11), 1011.

Qiao, J., Chen, Y., Mi, Y., Jin, H., Huang, T., Liu, L., ... & Zou, Z. (2020). NR5A2 synergizes with NCOA3 to induce breast cancer resistance to BET inhibitor by upregulating NRF2 to attenuate ferroptosis. Biochemical and Biophysical Research Communications, 530(2), 402-409.

Salazar-Silva, R., Dantas, V. L. G., Alves, L. U., Batissoco, A. C., Oiticica, J., Lawrence, E. A., ... & Mingroni-Netto, R. C. (2020). NCOA3 identified as a new candidate to explain autosomal dominant progressive hearing loss. Human Molecular Genetics, 29(22), 3691-3705.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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