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Mouse Anti-NCOA3 Recombinant Antibody (CBXS-3650) (CBMAB-S0929-CQ)

This product is a mouse antibody that recognizes NCOA3. The antibody CBXS-3650 can be used for immunoassay techniques such as: FC, IF, WB.
See all NCOA3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-3650
Antibody Isotype
IgG
Application
FC, IF, WB

Basic Information

Immunogen
Recombinant human SRC-3 polypeptide fragment corresponding to amino acids 1-250
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.01 M HEPES, 0.15 M NaCl, 0.1 mg/mL BSA, 50% glycerol, pH 7.5
Preservative
0.2% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nuclear Receptor Coactivator 3
Introduction
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Entrez Gene ID
8202
UniProt ID
Q9Y6Q9
Alternative Names
Nuclear Receptor Coactivator 3; Thyroid Hormone Receptor Activator Molecule 1; Receptor-Associated Coactivator 3; Class E Basic Helix-Loop-Helix Protein 42; Steroid Receptor Coactivator Protein 3; Amplified In Breast Cancer 1 Protein; CBP-Interacting Protein; EC 2.3.1.48; BHLHe42; TRAM-1; AIB-1; SRC-3; AIB1;
Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Biological Process
Cell dedifferentiation Source: UniProtKB
Cellular response to estradiol stimulus Source: BHF-UCL
Cellular response to hormone stimulus Source: GO_Central
Positive regulation of keratinocyte differentiation Source: UniProtKB
Positive regulation of stem cell population maintenance Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Receptor transactivation Source: UniProtKB
Regulation of stem cell division Source: UniProtKB
Cellular Location
Nucleus
Cytoplasm
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
PTM
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated
Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.

Kiliti, A. J., Sharif, G. M., Martin, M. B., Wellstein, A., & Riegel, A. T. (2023). AIB1/SRC-3/NCOA3 function in estrogen receptor alpha positive breast cancer. Frontiers in Endocrinology, 14, 1250218.

Li, X., Zhang, J., Wang, B., Chen, C., Zhang, E., Lv, Z., ... & Zhang, F. (2023). USP24-dependent stabilization of Runx2 recruits a p300/NCOA3 complex to transactivate ADAMTS genes and promote degeneration of intervertebral disc in chronic inflammation mice. Biology Direct, 18(1), 37.

Liu, D., Li, J., Hao, W., Lin, X., Xia, J., Zhu, J., ... & Yang, X. (2022). Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3. Frontiers in Veterinary Science, 9, 895190.

Li, Y., Liang, J., Dang, H., Zhang, R., Chen, P., & Shao, Y. (2022). NCOA3 is a critical oncogene in thyroid cancer via the modulation of major signaling pathways. Endocrine, 1-10.

Cui, Y., Fan, J., Shi, W., & Zhou, Z. (2022). Circ_0001667 knockdown blocks cancer progression and attenuates adriamycin resistance by depleting NCOA3 via releasing miR‐4458 in breast cancer. Drug Development Research, 83(1), 75-87.

de Semir, D., Bezrookove, V., Nosrati, M., Dar, A. A., Miller III, J. R., Leong, S. P., ... & Kashani-Sabet, M. (2021). Nuclear Receptor Coactivator NCOA3 Regulates UV Radiation–Induced DNA Damage and Melanoma Susceptibility. Cancer research, 81(11), 2956-2969.

Zheng, X., Jia, Y., Qiu, L., Zeng, X., Xu, L., Wei, M., ... & Han, J. (2020). A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator. Journal of Biological Chemistry, 295(6), 1474-1488.

Li, W., Yan, Y., Zheng, Z., Zhu, Q., Long, Q., Sui, S., ... & Li, L. (2020). Targeting the NCOA3-SP1-TERT axis for tumor growth in hepatocellular carcinoma. Cell death & disease, 11(11), 1011.

Qiao, J., Chen, Y., Mi, Y., Jin, H., Huang, T., Liu, L., ... & Zou, Z. (2020). NR5A2 synergizes with NCOA3 to induce breast cancer resistance to BET inhibitor by upregulating NRF2 to attenuate ferroptosis. Biochemical and Biophysical Research Communications, 530(2), 402-409.

Salazar-Silva, R., Dantas, V. L. G., Alves, L. U., Batissoco, A. C., Oiticica, J., Lawrence, E. A., ... & Mingroni-Netto, R. C. (2020). NCOA3 identified as a new candidate to explain autosomal dominant progressive hearing loss. Human Molecular Genetics, 29(22), 3691-3705.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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