Mouse Anti-NECAB2 Recombinant Antibody (CBWJN-0576) (CBMAB-N1717-WJ)

Name: Mouse Anti-NECAB2 Recombinant Antibody (CBWJN-0576)
SKU: CBMAB-N1717-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0576

Application

IHC

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 40% glycerol, pH 7.2

Preservative

0.02% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

N-Terminal EF-Hand Calcium Binding Protein 2

Introduction

The protein encoded by this gene is a neuronal calcium-binding protein that binds to and modulates the function of at least two receptors, adenosine A(2A) receptor and metabotropic glutamate receptor type 5. [provided by RefSeq, Jul 2016]

Entrez Gene ID

54550

UniProt ID

Q7Z6G3

Alternative Names

N-Terminal EF-Hand Calcium Binding Protein 2; Synaptotagmin-Interacting Protein 2; Neuronal Calcium-Binding Protein 2; EF-Hand Calcium-Binding Protein 2; Stip-2; EFCBP2; N-Terminal EF-Hand Calcium-Binding Protein 2; EF-Hand Calcium Binding Protein 2; Neuronal Calcium Binding 2;

Function

May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.

Biological Process

Negative regulation of G protein-coupled receptor internalization Source: UniProtKB
Positive regulation of adenosine receptor signaling pathway Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of glutamate receptor signaling pathway Source: UniProtKB
Positive regulation of protein localization to membrane Source: UniProtKB
Regulation of amyloid precursor protein biosynthetic process Source: GO_Central

Cellular Location

Cytoplasm
Plasma membrane
Cell membrane
Other locations
dendrite
axon
Note: Colocalizes with ADORA2A and/or mGluR5/GRM5 at the plasma membrane (PubMed:17689978, PubMed:19694902). Found in neuronal somata (PubMed:26843217). Detected in the cytoplasm of striatal neurons, at postsynaptic sites, filling dendritic shafts and spines, and at presynaptic sites, filling axon terminals (By similarity).

Bueno, D., Dey, P. N., Schacht, T., Wolf, C., Wüllner, V., Morpurgo, E., ... & Methner, A. (2023). NECAB2 is an endosomal protein important for striatal function. Free Radical Biology and Medicine, 208, 643-656.

Chen, Z., Long, H., Guo, J., Wang, Y., He, K., Tao, C., ... & Pi, Y. (2022). Autism-Risk Gene necab2 Regulates Psychomotor and Social Behavior as a Neuronal Modulator of mGluR1 Signaling. Frontiers in Molecular Neuroscience, 15, 901682.

Miczán, V., Kelemen, K., Glavinics, J. R., László, Z. I., Barti, B., Kenesei, K., ... & Katona, I. (2021). NECAB1 and NECAB2 are prevalent calcium-binding proteins of CB1/CCK-positive GABAergic interneurons. Cerebral Cortex, 31(3), 1786-1806.

Ma, Y., Deng, Q., Li, S., Chen, M., Jin, B., & Wang, M. (2021). TRPV1, Targeted by miR-338-3p, induces neuropathic pain by interacting with NECAB2. Journal of Molecular Neuroscience, 71, 55-65.

Bueno, D., Dey, P. N., Schacht, T., Wolf, C., Wüllner, V., Morpurgo, E., ... & Methner, A. (2021). NECAB2 participates in an endosomal pathway of mitochondrial stress response at striatal synapses. bioRxiv, 2021-02.

Morpurgo, E. (2021). NECAB2/MitoNEET Represents an Alternative Activity-triggered Mitochondrial Quality Control System (Doctoral dissertation, Johannes Gutenberg-Universität Mainz).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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