Mouse Anti-NECTIN3 Recombinant Antibody (CBT4559) (V2LY-0625-LY1190)

Name: Mouse Anti-NECTIN3 Recombinant Antibody (CBT4559)
SKU: V2LY-0625-LY1190
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT4559

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Purified recombinant fragment of human CD113 (AA: extra 282-404) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Nectin Cell Adhesion Molecule 3

Entrez Gene ID

25945

UniProt ID

Q9NQS3

Function

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Biological Process

Establishment of protein localization to plasma membrane Source: ARUK-UCL
Fertilization Source: Ensembl
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: GO_Central
Homophilic cell adhesion via plasma membrane adhesion molecules Source: HGNC-UCL
Lens morphogenesis in camera-type eye Source: Ensembl
Protein localization to cell junction Source: Ensembl
Retina morphogenesis in camera-type eye Source: Ensembl
Spermatid development Source: InterPro

Cellular Location

Plasma membrane
Cell membrane
postsynaptic cell membrane

Topology

Extracellular: 58-404
Helical: 405-425
Cytoplasmic: 426-549

More Infomation

References

Zhao, Y., Hong, X. H., Li, K., Li, Y. Q., Li, Y. Q., He, S. W., ... & Chen, Y. P. (2020). ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin‐3 and NRXN3. Cancer Communications, 40(12), 721-737.

Hirabayashi, K., Tajiri, T., Bosch, D. E., Morimachi, M., Miyaoka, M., Inomoto, C., ... & Yeh, M. M. (2020). Loss of nectin‐3 expression as a marker of tumor aggressiveness in pancreatic neuroendocrine tumor. Pathology International, 70(2), 84-91.

Xu, F., Si, X., Wang, J., Yang, A., Qin, T., & Yang, Y. (2019). Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells. Biomedicine & Pharmacotherapy, 110, 139-144.

Liu, R., Wang, H., Wang, H. L., Sun, Y. X., Su, Y. A., Wang, X. D., ... & Si, T. M. (2019). Postnatal nectin‐3 knockdown induces structural abnormalities of hippocampal principal neurons and memory deficits in adult mice. Hippocampus, 29(11), 1063-1074.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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