Mouse Anti-NEDD1 Recombinant Antibody (3H7) (CBMAB-N1745-WJ)

This product is a Mouse antibody that recognizes NEDD1. The antibody 3H7 can be used for immunoassay techniques such as: ELISA, WB.
See all NEDD1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3H7

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

neural precursor cell expressed, developmentally down-regulated 1

Introduction

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Entrez Gene ID

121441

UniProt ID

Q8NHV4

Alternative Names

Neural Precursor Cell Expressed, Developmentally Down-Regulated 1; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1; NEDD-1; Protein NEDD1; TUBGCP7; GCP-WD;

Function

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Protein localization to centrosome Source: GO_Central

Cellular Location

Centrosome

PTM

During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation.

References

Timón Pérez, K., Scrofani, J., & Vernos, I. (2022). NEDD1-S411 phosphorylation plays a critical function in the coordination of microtubule nucleation during mitosis. Biology Open, 11(11), bio059474.

Zhou, X., Ding, X., Yu, J., Shi, X., Li, K., Ma, S., ... & Han, Y. (2022). Novel biomarker NEDD1 and its analysis in hepatocellular carcinoma.

Chi, W., Wang, G., Xin, G., Jiang, Q., & Zhang, C. (2021). PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations. Journal of Cell Biology, 220(1).

Courthéoux, T., Reboutier, D., Vazeille, T., Cremet, J. Y., Benaud, C., Vernos, I., & Prigent, C. (2019). Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A. Journal of Cell Science, 132(10), jcs231118.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NEDD1 Recombinant Antibody (7D10)

Mouse Anti-NEDD1 Recombinant Antibody (H-3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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