Mouse Anti-NEDD1 Recombinant Antibody (7D10) (CBMAB-N1746-WJ)

Name: Mouse Anti-NEDD1 Recombinant Antibody (7D10)
SKU: CBMAB-N1746-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7D10

Application

ELISA, IHC, WB

Specificity

Human, Mouse

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

neural precursor cell expressed, developmentally down-regulated 1

Introduction

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Entrez Gene ID

Human	121441
Mouse	17997

UniProt ID

Human	Q8NHV4
Mouse	P33215

Alternative Names

Neural Precursor Cell Expressed, Developmentally Down-Regulated 1; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1; NEDD-1; Protein NEDD1; TUBGCP7; GCP-WD;

Function

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Protein localization to centrosome Source: GO_Central

Cellular Location

Centrosome

PTM

During mitosis, prior phosphorylation on Thr-550 by CDK1 promotes subsequent phosphorylation by PLK1 on Thr-382, Ser-397, Ser-426 and Ser-637. Phosphorylated NEDD1 can interact with gamma-tubulin for targeting the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation.

Timón Pérez, K., Scrofani, J., & Vernos, I. (2022). NEDD1-S411 phosphorylation plays a critical function in the coordination of microtubule nucleation during mitosis. Biology Open, 11(11), bio059474.

Zhou, X., Ding, X., Yu, J., Shi, X., Li, K., Ma, S., ... & Han, Y. (2022). Novel biomarker NEDD1 and its analysis in hepatocellular carcinoma.

Chi, W., Wang, G., Xin, G., Jiang, Q., & Zhang, C. (2021). PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations. Journal of Cell Biology, 220(1).

Courthéoux, T., Reboutier, D., Vazeille, T., Cremet, J. Y., Benaud, C., Vernos, I., & Prigent, C. (2019). Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A. Journal of Cell Science, 132(10), jcs231118.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-NEDD1 Recombinant Antibody (3H7) (CAT#: CBMAB-N1745-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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