Mouse Anti-NEIL1 Recombinant Antibody (1D1) (CBMAB-N1904-WJ)

Name: Mouse Anti-NEIL1 Recombinant Antibody (1D1)
SKU: CBMAB-N1904-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D1

Application

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nei Like DNA Glycosylase 1

Introduction

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Entrez Gene ID

79661

UniProt ID

Q96FI4

Alternative Names

Nei Like DNA Glycosylase 1; DNA-(Apurinic Or Apyrimidinic Site) Lyase Neil1; DNA Endonuclease Eight-Like Glycosylase 1; DNA Glycosylase/AP Lyase Neil1; Endonuclease VIII-Like 1; Nei-Like Protein 1; Nei Homolog 1; FPG1; NEH1;

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.

Biological Process

Base-excision repair Source: UniProtKB
Base-excision repair, gap-filling Source: Reactome
Depyrimidination Source: Reactome
Negative regulation of nuclease activity Source: UniProtKB
Response to oxidative stress Source: UniProtKB

Cellular Location

Nucleus
Cytoskeleton
centrosome
Other locations
Chromosome
Note: During mitosis, associates with centrosomes and condensed chromatin.

Cao, J. H., Cao, C. H., Lin, J. L., Li, S. Y., He, L. J., Han, K., ... & Wang, F. W. (2024). NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1. Cell Reports, 43(1), 113654-113654.

Lloyd, R. S. (2022). Complex roles of NEIL1 and OGG1: insights gained from murine knockouts and human polymorphic variants. DNA, 2(4), 279-301.

Lotsof, E. R., Krajewski, A. E., Anderson-Steele, B., Rogers, J., Zhang, L., Yeo, J., ... & David, S. S. (2022). NEIL1 recoding due to RNA editing impacts lesion-specific recognition and excision. Journal of the American Chemical Society, 144(32), 14578-14589.

Hanna, B. M., Michel, M., Helleday, T., & Mortusewicz, O. (2021). NEIL1 and NEIL2 Are Recruited as Potential Backup for OGG1 upon OGG1 Depletion or Inhibition by TH5487. International Journal of Molecular Sciences, 22(9), 4542.

Yeo, J., Lotsof, E. R., Anderson-Steele, B. M., & David, S. S. (2021). RNA editing of the human DNA glycosylase NEIL1 alters its removal of 5-hydroxyuracil lesions in DNA. Biochemistry, 60(19), 1485-1497.

Minko, I. G., Vartanian, V. L., Tozaki, N. N., Coskun, E., Coskun, S. H., Jaruga, P., ... & Lloyd, R. S. (2020). Recognition of DNA adducts by edited and unedited forms of DNA glycosylase NEIL1. DNA repair, 85, 102741.

Albelazi, M. S., Martin, P. R., Mohammed, S., Mutti, L., Parsons, J. L., & Elder, R. H. (2019). The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks. Genes, 10(4), 315.

Kladova, O. A., Grin, I. R., Fedorova, O. S., Kuznetsov, N. A., & Zharkov, D. O. (2019). Conformational dynamics of damage processing by human DNA glycosylase NEIL1. Journal of molecular biology, 431(6), 1098-1112.

Yang, B., Figueroa, D. M., Hou, Y., Babbar, M., Baringer, S. L., Croteau, D. L., & Bohr, V. A. (2019). NEIL1 stimulates neurogenesis and suppresses neuroinflammation after stress. Free Radical Biology and Medicine, 141, 47-58.

Han, D., Schomacher, L., Schüle, K. M., Mallick, M., Musheev, M. U., Karaulanov, E., ... & Niehrs, C. (2019). NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress. elife, 8, e49044.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

NEIL1 Matched Antibody Pair (786) (CAT#: APMAB-786LY)

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NEIL1 Matched Antibody Pair (786) (CAT#: APMAB-786LY)

Mouse Anti-NEIL1 Recombinant Antibody (D-1) (CAT#: CBMAB-N1906-WJ)

Mouse Anti-NEIL1 Recombinant Antibody (D-4) (CAT#: CBMAB-N1907-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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