Human Recombinant NEIL1 protein, His Tag (V2LY-0526-LY5732)

Name: Human Recombinant NEIL1 protein, His Tag
SKU: V2LY-0526-LY5732
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NEIL1 protein, His Tag consist of Amino Acid: 1-390 and predicts a molecular mass of 45 kDa.

Molecule Mass

45 kDa

Sequence

Amino Acid: 1-390

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>84% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Nei Like DNA Glycosylase 1

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.

Biological Process

Base-excision repair Source: UniProtKB
Base-excision repair, gap-filling Source: Reactome
Depyrimidination Source: Reactome
Negative regulation of nuclease activity Source: UniProtKB
Response to oxidative stress Source: UniProtKB

Cellular Location

Nucleus
Cytoskeleton
centrosome
Other locations
Chromosome
Note: During mitosis, associates with centrosomes and condensed chromatin.

Cao, J. H., Cao, C. H., Lin, J. L., Li, S. Y., He, L. J., Han, K., ... & Wang, F. W. (2024). NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1. Cell Reports, 43(1), 113654-113654.

Lloyd, R. S. (2022). Complex roles of NEIL1 and OGG1: insights gained from murine knockouts and human polymorphic variants. DNA, 2(4), 279-301.

Lotsof, E. R., Krajewski, A. E., Anderson-Steele, B., Rogers, J., Zhang, L., Yeo, J., ... & David, S. S. (2022). NEIL1 recoding due to RNA editing impacts lesion-specific recognition and excision. Journal of the American Chemical Society, 144(32), 14578-14589.

Hanna, B. M., Michel, M., Helleday, T., & Mortusewicz, O. (2021). NEIL1 and NEIL2 Are Recruited as Potential Backup for OGG1 upon OGG1 Depletion or Inhibition by TH5487. International Journal of Molecular Sciences, 22(9), 4542.

Yeo, J., Lotsof, E. R., Anderson-Steele, B. M., & David, S. S. (2021). RNA editing of the human DNA glycosylase NEIL1 alters its removal of 5-hydroxyuracil lesions in DNA. Biochemistry, 60(19), 1485-1497.

Minko, I. G., Vartanian, V. L., Tozaki, N. N., Coskun, E., Coskun, S. H., Jaruga, P., ... & Lloyd, R. S. (2020). Recognition of DNA adducts by edited and unedited forms of DNA glycosylase NEIL1. DNA repair, 85, 102741.

Albelazi, M. S., Martin, P. R., Mohammed, S., Mutti, L., Parsons, J. L., & Elder, R. H. (2019). The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks. Genes, 10(4), 315.

Kladova, O. A., Grin, I. R., Fedorova, O. S., Kuznetsov, N. A., & Zharkov, D. O. (2019). Conformational dynamics of damage processing by human DNA glycosylase NEIL1. Journal of molecular biology, 431(6), 1098-1112.

Yang, B., Figueroa, D. M., Hou, Y., Babbar, M., Baringer, S. L., Croteau, D. L., & Bohr, V. A. (2019). NEIL1 stimulates neurogenesis and suppresses neuroinflammation after stress. Free Radical Biology and Medicine, 141, 47-58.

Han, D., Schomacher, L., Schüle, K. M., Mallick, M., Musheev, M. U., Karaulanov, E., ... & Niehrs, C. (2019). NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress. elife, 8, e49044.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

NEIL1 Matched Antibody Pair (786) (CAT#: APMAB-786LY)

Antibody Pairs

NEIL1 Matched Antibody Pair (786) (CAT#: APMAB-786LY)

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Mouse Anti-NEIL1 Recombinant Antibody (D-1) (CAT#: CBMAB-N1906-WJ)

NEIL1 Matched Antibody Pair (786) (CAT#: APMAB-786LY)

Mouse Anti-NEIL1 Recombinant Antibody (D-4) (CAT#: CBMAB-N1907-WJ)

Mouse Anti-NEIL1 Recombinant Antibody (1D1) (CAT#: CBMAB-N1904-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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