Mouse Anti-NEK3 Recombinant Antibody (2F8) (CBMAB-N1929-WJ)

Name: Mouse Anti-NEK3 Recombinant Antibody (2F8)
SKU: CBMAB-N1929-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F8

Application

ELISA, IF, WB

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NIMA Related Kinase 3

Introduction

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Entrez Gene ID

4752

UniProt ID

P51956

Alternative Names

NIMA Related Kinase 3; NIMA (Never In Mitosis Gene A)-Related Kinase 3; Never In Mitosis A-Related Kinase 3; Phosphorylase B Kinase Kinase; NimA-Related Protein Kinase 3; Hydroxyalkyl-Protein Kinase; Glycogen Synthase A Kinase;

Function

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

Biological Process

Cell division Source: UniProtKB-KW
Establishment of cell polarity Source: Ensembl
Mitotic cell cycle Source: UniProtKB
Neuron projection morphogenesis Source: Ensembl
Protein phosphorylation Source: UniProtKB
Regulation of tubulin deacetylation Source: Ensembl

Cellular Location

Cytoplasm
Other locations
axon

PTM

Phosphorylation at Thr-479 regulates its catalytic activity.

Zhang, Y., Chen, W., Zeng, W., Lu, Z., & Zhou, X. (2020). Biallelic loss of function NEK3 mutations deacetylate α-tubulin and downregulate NUP205 that predispose individuals to cilia-related abnormal cardiac left–right patterning. Cell Death & Disease, 11(11), 1005.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NEK3 Matched Antibody Pair (791) (CAT#: APMAB-791LY)

Related Products

NEK3 Matched Antibody Pair (791) (CAT#: APMAB-791LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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