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Rabbit Anti-NEK7 Recombinant Antibody (H.691.4) (CBMAB-N1947-WJ)

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Basic Information

Host Animal
Rabbit
Clone
H.691.4
Application
WB
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.01M HEPES, 0.15M NaCl, BSA, 50% glycerol, pH 7.5
Preservative
0.02% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
NIMA Related Kinase 7
Introduction
NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]
Entrez Gene ID
140609
UniProt ID
Q8TDX7
Alternative Names
NIMA Related Kinase 7; EC 2.7.11.1; NIMA (Never In Mitosis Gene A)-Related Kinase 7; Never In Mitosis A-Related Kinase 7; NimA-Related Protein Kinase 7;
Function
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132).

Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596).

Phosphorylates RPS6KB1 (By similarity).

Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).

Essential protein for NLRP3 inflammasome assembly and activation that acts downstream stimuli such as K+ efflux. Dispensable for the induction of core inflammasome components, but necessary for subsequent formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division. Exerts its effects on the NLRP3 inflammasome independently of its kinase activity (PubMed:26642356).
Biological Process
Cellular response to potassium ion Source: UniProtKB
Positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
Positive regulation of telomerase activity Source: BHF-UCL
Positive regulation of telomere capping Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein phosphorylation Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Spindle assembly Source: UniProtKB
Cellular Location
Cytoplasm
Nucleus
Cytoskeleton
spindle pole
centrosome
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).
PTM
Phosphorylation at Ser-195 required for its activation.

Zhang, H., & Li, B. (2023). NIMA-related kinase 6 as an effective target inhibits the hepatocarcinogenesis and progression of hepatocellular carcinoma. Heliyon, 9(6).

Sun, Z., Wang, Q., Sun, L., Wu, M., Li, S., Hua, H., ... & Jia, Z. (2022). Acetaminophen-induced reduction of NIMA-related kinase 7 expression exacerbates acute liver injury. JHEP Reports, 4(10), 100545.

Wu, G., Zhang, D., Yang, L., Wu, Q., & Yuan, L. (2022). MicroRNA-200c-5p targets NIMA Related Kinase 7 (NEK7) to inhibit NOD-like receptor 3 (NLRP3) inflammasome activation, MODE-K cell pyroptosis, and inflammatory bowel disease in mice. Molecular Immunology, 146, 57-68.

Ji, X., Song, Z., He, J., Guo, S., Chen, Y., Wang, H., ... & Liu, J. (2021). NIMA-related kinase 7 amplifies NLRP3 inflammasome pro-inflammatory signaling in microglia/macrophages and mice models of spinal cord injury. Experimental Cell Research, 398(2), 112418.

Zhang, X. J., Wang, T. T., Pu, Y. K., Zeng, L., Zhang, R. H., Li, X. L., ... & Xiao, W. L. (2021). Optimized Expression of Recombinant Human NIMA-related Kinase 7 (NEK7) with A Higher Purity in Escherichia coli. Protein and Peptide Letters, 28(12), 1391-1397.

Sun, Z., Gong, W., Zhang, Y., & Jia, Z. (2020). Physiological and pathological roles of mammalian NEK7. Frontiers in physiology, 11, 606996.

Zhou, X., Zhang, P., Wang, Q., Ji, N., Xia, S., Ding, Y., & Wang, Q. (2019). Metformin ameliorates experimental diabetic periodontitis independently of mammalian target of rapamycin (mTOR) inhibition by reducing NIMA‐related kinase 7 (Nek7) expression. Journal of periodontology, 90(9), 1032-1042.

Perez, A., de Souza, E., & Kobarg, J. (2019). NIMA-related kinase 7 interacts with Mat1 and is involved in the UV-induced DNA Damage Response. Journal of Integrated OMICS, 9(2).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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