Mouse Recombinant NEK7 protein, GST Tag (V2LY-0526-LY8697)

Name: Mouse Recombinant NEK7 protein, GST Tag
SKU: V2LY-0526-LY8697
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Mouse

Tag

GST Tag

Protein Construction

This product is Mouse Recombinant NEK7 protein, GST Tag consist of Amino Acid: 1-302 and predicts a molecular mass of 60 kDa.

Molecule Mass

60 kDa

Sequence

Amino Acid: 1-302

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

NIMA Related Kinase 7

Function

Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132).

Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596).

Phosphorylates RPS6KB1 (By similarity).

Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).

Essential protein for NLRP3 inflammasome assembly and activation that acts downstream stimuli such as K+ efflux. Dispensable for the induction of core inflammasome components, but necessary for subsequent formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division. Exerts its effects on the NLRP3 inflammasome independently of its kinase activity (PubMed:26642356).

Biological Process

Cellular response to potassium ion Source: UniProtKB
Positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
Positive regulation of telomerase activity Source: BHF-UCL
Positive regulation of telomere capping Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein phosphorylation Source: UniProtKB
Regulation of mitotic cell cycle Source: UniProtKB
Spindle assembly Source: UniProtKB

Cellular Location

Cytoplasm
Nucleus
Cytoskeleton
spindle pole
centrosome
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).

PTM

Phosphorylation at Ser-195 required for its activation.

Zhang, H., & Li, B. (2023). NIMA-related kinase 6 as an effective target inhibits the hepatocarcinogenesis and progression of hepatocellular carcinoma. Heliyon, 9(6).

Sun, Z., Wang, Q., Sun, L., Wu, M., Li, S., Hua, H., ... & Jia, Z. (2022). Acetaminophen-induced reduction of NIMA-related kinase 7 expression exacerbates acute liver injury. JHEP Reports, 4(10), 100545.

Wu, G., Zhang, D., Yang, L., Wu, Q., & Yuan, L. (2022). MicroRNA-200c-5p targets NIMA Related Kinase 7 (NEK7) to inhibit NOD-like receptor 3 (NLRP3) inflammasome activation, MODE-K cell pyroptosis, and inflammatory bowel disease in mice. Molecular Immunology, 146, 57-68.

Ji, X., Song, Z., He, J., Guo, S., Chen, Y., Wang, H., ... & Liu, J. (2021). NIMA-related kinase 7 amplifies NLRP3 inflammasome pro-inflammatory signaling in microglia/macrophages and mice models of spinal cord injury. Experimental Cell Research, 398(2), 112418.

Zhang, X. J., Wang, T. T., Pu, Y. K., Zeng, L., Zhang, R. H., Li, X. L., ... & Xiao, W. L. (2021). Optimized Expression of Recombinant Human NIMA-related Kinase 7 (NEK7) with A Higher Purity in Escherichia coli. Protein and Peptide Letters, 28(12), 1391-1397.

Sun, Z., Gong, W., Zhang, Y., & Jia, Z. (2020). Physiological and pathological roles of mammalian NEK7. Frontiers in physiology, 11, 606996.

Zhou, X., Zhang, P., Wang, Q., Ji, N., Xia, S., Ding, Y., & Wang, Q. (2019). Metformin ameliorates experimental diabetic periodontitis independently of mammalian target of rapamycin (mTOR) inhibition by reducing NIMA‐related kinase 7 (Nek7) expression. Journal of periodontology, 90(9), 1032-1042.

Perez, A., de Souza, E., & Kobarg, J. (2019). NIMA-related kinase 7 interacts with Mat1 and is involved in the UV-induced DNA Damage Response. Journal of Integrated OMICS, 9(2).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY1610)

Human Recombinant NEK7, Active protein, GST Tag (CAT#: V2LY-0526-LY5741)

Human Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY5742)

Rabbit Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY9138)

Rat Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY9529)

Related Products

Cynomolgus Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY1610)

Human Recombinant NEK7, Active protein, GST Tag (CAT#: V2LY-0526-LY5741)

Rabbit Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY9138)

Rabbit Anti-NEK7 Recombinant Antibody (CBWJN-1322) (CAT#: CBMAB-N1946-WJ)

Human Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY5742)

Rat Recombinant NEK7 protein, GST Tag (CAT#: V2LY-0526-LY9529)

Rabbit Anti-NEK7 Recombinant Antibody (H.691.4) (CAT#: CBMAB-N1947-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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