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Human Anti-NES Recombinant Antibody (196908) (CBMAB-0512CQ)

This product is a human antibody that recognizes NES. The antibody 196908 can be used for immunoassay techniques such as: ELISA, FC, ICC, IF, IHC-FoFr, IHC-Fr, IP, WB.
See all NES antibodies
Published Data
Fig.1 Immunofluorescence imaging of differentiated cells with ectoderm (NESTIN and PAX6) markers.
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Fig.2 Representative confocal images showing the colocalization of the human nuclear antigen (huN) with neural phenotype marker Nestin in transplanted hNSCs at 40DAT.
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Summary

Host Animal
Human
Specificity
Human
Clone
196908
Antibody Isotype
IgG1
Application
ELISA, FC, ICC, IF, IHC-FoFr, IHC-Fr, IP, WB

Basic Information

Immunogen
Human Nestin fragment aa residues 618-1618
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nestin
Introduction
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments. GO annotations related to this gene include structural molecule activity and intermediate filament binding. An important paralog of this gene is SYNM.
Entrez Gene ID
10763
UniProt ID
P48681
Alternative Names
Nbla00170
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Biological Process
Brain development Source: UniProtKB
Cell projection morphogenesis Source: Ensembl
Central nervous system development Source: UniProtKB
Embryonic camera-type eye development Source: UniProtKB
G2/M transition of mitotic cell cycle Source: DFLAT
Negative regulation of catalytic activity Source: UniProtKB
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of protein binding Source: UniProtKB
Positive regulation of intermediate filament depolymerization Source: UniProtKB
Positive regulation of neural precursor cell proliferation Source: UniProtKB
Stem cell proliferation Source: DFLAT
Cellular Location
Cytoskeleton
intermediate filament
intermediate filament cytoskeleton
Other locations
cytoplasm
PTM
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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