Mouse Anti-NEU2 Recombinant Antibody (5E1) (CBMAB-N2020-WJ)

Name: Mouse Anti-NEU2 Recombinant Antibody (5E1)
SKU: CBMAB-N2020-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5E1

Application

WB, IF

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.33 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

sialidase 2 (cytosolic sialidase)

Introduction

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4759

UniProt ID

Q9Y3R4

Alternative Names

Neuraminidase 2; N-Acetyl-Alpha-Neuraminidase 2; Sialidase 2 (Cytosolic Sialidase); Cytosolic Sialidase; EC 3.2.1.18; Neuraminidase 2 (Cytosolic Sialidase); Sialidase-2; SIAL2;

Function

Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides (PubMed:14613940, PubMed:22228546).

Recognizes sialyl linkage positions of the glycan moiety as well as the supramolecular organization of the sialoglycoconjugate. Displays preference for alpha-(2->3)-sialylated GD1a and GT1B gangliosides over alpha-(2->8)-sialylated GD1b, in both monomeric forms and micelles. Hydrolyzes monomeric GM1 ganglioside, but has no activity toward the miscellar form (PubMed:14613940).

Has lower sialidase activity for glycoproteins such as fetuin and TF/transferrin that carry a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. Cleaves milk oligosaccharide alpha-(2->3)-sialyllactose, but is inactive toward alpha-(2->6)-sialyllactose isomer. Has no activity toward colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues (PubMed:14613940).

Biological Process

Cellular oligosaccharide catabolic process Source: CAFA
Ganglioside catabolic process Source: UniProtKB
Glycoprotein catabolic process Source: UniProtKB
Glycosphingolipid metabolic process Source: Reactome
Oligosaccharide catabolic process Source: UniProtKB

Cellular Location

Cytosol

Li, Z., Wu, N., Wang, J., Yue, Y., Geng, L., & Zhang, Q. (2023). Low molecular weight fucoidan (LMWF) restores diabetic endothelial glycocalyx by targeting Neuraminidase2 (NEU2): a new therapy target in glycocalyx shedding. British Journal of Pharmacology.

van der Wal, D. E., Davis, A. M., Mach, M., & Marks, D. C. (2020). The role of neuraminidase 1 and 2 in glycoprotein Ibα-mediated integrin αIIbβ3 activation. Haematologica, 105(4), 1081.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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