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Mouse Anti-NFIL3 Antibody (2B5) (CBMAB-0724-YC)

Provided herein are mouse monoclonal antibodies against Human NFIL3. The antibody clone 2B5 can be used for immunoassay techniques, such as ChIP, IP and MA.
See all NFIL3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B5
Antibody Isotype
IgG1
Application
ChIP, IP, MA

Basic Information

Immunogen
Recombinant peptide
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nuclear Factor, Interleukin 3 Regulated
Introduction
NFIL3 (nuclear factor, interleukin 3 regulated) is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. NFIL3 represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm.
Entrez Gene ID
4783
UniProt ID
Q16649
Alternative Names
E4BP4; IL3BP1; NF-IL3A; NFIL3A
Function
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity).
Represses transcriptional activity of PER1 (By similarity).
Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity).
Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity).
Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity).
Protects pro-B cells from programmed cell death (By similarity).
Represses the transcription of CYP2A5 (By similarity).
Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Required for the development of natural killer cell precursors (By similarity).
Biological Process
Cellular response to interleukin-4IEA:Ensembl
Circadian rhythmManual Assertion Based On ExperimentIBA:GO_Central
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Natural killer cell differentiationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:GO_Central
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Regulation of transcription by RNA polymerase II2 PublicationsIC:ComplexPortal
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus

Chen, S., Lei, M., Liu, K., & Min, J. (2024). Structural basis for specific DNA sequence recognition by the transcription factor NFIL3. Journal of Biological Chemistry, 105776.

Park, J., Cho, Y., Yang, D., Yang, H., Lee, D., Kubo, M., & Kang, S. J. (2024). The transcription factor NFIL3/E4BP4 regulates the developmental stage–specific acquisition of basophil function. Journal of Allergy and Clinical Immunology, 153(1), 132-145.

Zeng, G. G., Zhou, J., Jiang, W. L., Yu, J., Nie, G. Y., Li, J., ... & Tang, C. K. (2023). A potential role of NFIL3 in atherosclerosis. Current problems in cardiology, 102096.

Yang, W., Li, J., Zhang, M., Yu, H., Zhuang, Y., Zhao, L., ... & Li, X. (2022). Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription. Journal of Experimental & Clinical Cancer Research, 41(1), 67.

Zhuo, H., & Liu, J. (2022). Nuclear Factor Interleukin 3 (NFIL3) Participates in regulation of the NF-ΚB-mediated inflammation and antioxidant system in Litopenaeus vannamei under ammonia-n stress. Fish & Shellfish Immunology, 131, 1192-1205.

Kubo, M. (2020). Diurnal rhythmicity programs of microbiota and transcriptional oscillation of circadian regulator, NFIL3. Frontiers in Immunology, 11, 552188.

Hariri, H., Pellicelli, M., & St-Arnaud, R. (2020). Nfil3, a target of the NACA transcriptional coregulator, affects osteoblast and osteocyte gene expression differentially. Bone, 141, 115624.

Bagadia, P., Huang, X., Liu, T. T., Durai, V., Grajales-Reyes, G. E., Nitschké, M., ... & Murphy, K. M. (2019). An Nfil3–Zeb2–Id2 pathway imposes Irf8 enhancer switching during cDC1 development. Nature immunology, 20(9), 1174-1185.

Kim, H. S., Sohn, H., Jang, S. W., & Lee, G. R. (2019). The transcription factor NFIL3 controls regulatory T-cell function and stability. Experimental & molecular medicine, 51(7), 1-15.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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