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Rabbit Anti-NFIL3 Recombinant Antibody (D5K8O) (CBMAB-CP0526-LY)

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Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
D5K8O
Application
WB, IP
Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4/NFIL3 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Nuclear Factor, Interleukin 3 Regulated
Introduction
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
Entrez Gene ID
Human4783
Mouse18030
Rat114519
UniProt ID
HumanQ16649
MouseO08750
RatQ6IMZ0
Alternative Names
Nuclear Factor; Interleukin 3 Regulated; Interleukin-3 Promoter Transcriptional Activator; Transcriptional Activator NF-IL3A; Interleukin-3-Binding Protein 1; E4 Promoter-Binding Protein 4; IL3BP1;
Function
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity).
Represses transcriptional activity of PER1 (By similarity).
Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity).
Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity).
Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity).
Protects pro-B cells from programmed cell death (By similarity).
Represses the transcription of CYP2A5 (By similarity).
Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity).
Required for the development of natural killer cell precursors (By similarity).
Biological Process
Cellular response to interleukin-4IEA:Ensembl
Circadian rhythmManual Assertion Based On ExperimentIBA:GO_Central
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Natural killer cell differentiationISS:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:BHF-UCL
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:GO_Central
Positive regulation of gene expressionIEA:Ensembl
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Regulation of transcription by RNA polymerase II2 PublicationsIC:ComplexPortal
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus

Chen, S., Lei, M., Liu, K., & Min, J. (2024). Structural basis for specific DNA sequence recognition by the transcription factor NFIL3. Journal of Biological Chemistry, 105776.

Park, J., Cho, Y., Yang, D., Yang, H., Lee, D., Kubo, M., & Kang, S. J. (2024). The transcription factor NFIL3/E4BP4 regulates the developmental stage–specific acquisition of basophil function. Journal of Allergy and Clinical Immunology, 153(1), 132-145.

Zeng, G. G., Zhou, J., Jiang, W. L., Yu, J., Nie, G. Y., Li, J., ... & Tang, C. K. (2023). A potential role of NFIL3 in atherosclerosis. Current problems in cardiology, 102096.

Yang, W., Li, J., Zhang, M., Yu, H., Zhuang, Y., Zhao, L., ... & Li, X. (2022). Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription. Journal of Experimental & Clinical Cancer Research, 41(1), 67.

Zhuo, H., & Liu, J. (2022). Nuclear Factor Interleukin 3 (NFIL3) Participates in regulation of the NF-ΚB-mediated inflammation and antioxidant system in Litopenaeus vannamei under ammonia-n stress. Fish & Shellfish Immunology, 131, 1192-1205.

Kubo, M. (2020). Diurnal rhythmicity programs of microbiota and transcriptional oscillation of circadian regulator, NFIL3. Frontiers in Immunology, 11, 552188.

Hariri, H., Pellicelli, M., & St-Arnaud, R. (2020). Nfil3, a target of the NACA transcriptional coregulator, affects osteoblast and osteocyte gene expression differentially. Bone, 141, 115624.

Bagadia, P., Huang, X., Liu, T. T., Durai, V., Grajales-Reyes, G. E., Nitschké, M., ... & Murphy, K. M. (2019). An Nfil3–Zeb2–Id2 pathway imposes Irf8 enhancer switching during cDC1 development. Nature immunology, 20(9), 1174-1185.

Kim, H. S., Sohn, H., Jang, S. W., & Lee, G. R. (2019). The transcription factor NFIL3 controls regulatory T-cell function and stability. Experimental & molecular medicine, 51(7), 1-15.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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