Rabbit Anti-NFKBIZ Recombinant Antibody (D4I7C) (CBMAB-CP1132-LY)

Name: Rabbit Anti-NFKBIZ Recombinant Antibody (D4I7C)
SKU: CBMAB-CP1132-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D4I7C

Application

WB, IP, CIP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly108 of mouse IκB-ζ protein.

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

NFKB Inhibitor Zeta

Introduction

This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

80859

UniProt ID

Q9EST8

Alternative Names

AA408868; INAP; Mail

Function

Involved in regulation of NF-kappa-B transcription factor complexes (PubMed:16513645, PubMed:16622025).
Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation (PubMed:16513645).
Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer (PubMed:16513645).
Seems also to activate NF-kappa-B-mediated transcription (PubMed:16622025).
In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters (PubMed:16622025).
Promotes transcription of LCN2 and DEFB4 (PubMed:16622025).
Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS (By similarity).
Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling (By similarity).
Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR) (By similarity).

Biological Process

Inflammatory responseIEA:Ensembl
Positive regulation of inflammatory responseManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of T-helper 17 cell differentiationISS:UniProtKB
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
T cell receptor signaling pathwayISS:UniProtKB

Cellular Location

Nucleus
Aggregated in dot-like structures (PubMed:16513645).
Colocalizes with NCOR2 (PubMed:16513645).

Muromoto, R., Sato, A., Komori, Y., Nariya, K., Kitai, Y., Kashiwakura, J. I., & Matsuda, T. (2022). Regulation of NFKBIZ gene promoter activity by STAT3, C/EBPβ, and STAT1. Biochemical and Biophysical Research Communications, 613, 61-66.

Camblor, D. G., Miranda, D., Albaiceta, G. M., Amado-Rodríguez, L., Cuesta-Llavona, E., Vázquez-Coto, D., ... & Coto, E. (2022). Genetic variants in the NF-κB signaling pathway (NFKB1, NFKBIA, NFKBIZ) and risk of critical outcome among COVID-19 patients. Human immunology, 83(8-9), 613-617.

Xu, T., Rao, T., Yu, W. M., Ning, J. Z., Yu, X., Zhu, S. M., ... & Cheng, F. (2021). Upregulation of NFKBIZ affects bladder cancer progression via the PTEN/PI3K/Akt signaling pathway. International Journal of Molecular Medicine, 47(6), 1-12.

Mandal, A., Kumbhojkar, N., Reilly, C., Dharamdasani, V., Ukidve, A., Ingber, D. E., & Mitragotri, S. (2020). Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations. Science advances, 6(30), eabb6049.

Kakiuchi, N., Yoshida, K., Uchino, M., Kihara, T., Akaki, K., Inoue, Y., ... & Ogawa, S. (2020). Frequent mutations that converge on the NFKBIZ pathway in ulcerative colitis. Nature, 577(7789), 260-265.

Liu, Z., Tang, C., He, L., Yang, D., Cai, J., Zhu, J., ... & Dong, Z. (2020). The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury. JCI insight, 5(24).

Slowikowski, K., Nguyen, H. N., Noss, E. H., Simmons, D. P., Mizoguchi, F., Watts, G. F., ... & Raychaudhuri, S. (2020). CUX1 and IκBζ (NFKBIZ) mediate the synergistic inflammatory response to TNF and IL-17A in stromal fibroblasts. Proceedings of the National Academy of Sciences, 117(10), 5532-5541.

Coto, E., Reguero, J. R., Avanzas, P., Pascual, I., Martín, M., Hevia, S., ... & Gómez, J. (2019). Gene variants in the NF-KB pathway (NFKB1, NFKBIA, NFKBIZ) and risk for early-onset coronary artery disease. Immunology letters, 208, 39-43.

Coto-Segura, P., González-Lara, L., Batalla, A., Eiris, N., Queiro, R., & Coto, E. (2019). NFKBIZ and Cw6 in adalimumab response among psoriasis patients: genetic association and alternative transcript analysis. Molecular Diagnosis & Therapy, 23, 627-633.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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