Mouse Anti-NFS1 Recombinant Antibody (1D5) (CBMAB-N2321-WJ)

Name: Mouse Anti-NFS1 Recombinant Antibody (1D5)
SKU: CBMAB-N2321-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D5

Application

ELISA, WB

Specificity

Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NFS1, Cysteine Desulfurase

Introduction

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]

Entrez Gene ID

9054

UniProt ID

Q9Y697

Alternative Names

NFS1, Cysteine Desulfurase; Nitrogen Fixation 1 (S. Cerevisiae, Homolog); EC 2.8.1.7; NIFS; NFS1 Nitrogen Fixation 1 Homolog (S. Cerevisiae); Nitrogen-Fixing Bacteria S-Like Protein;

Function

Catalyzes the removal of elemental sulfur from cysteine to produce alanine (PubMed:18650437).
It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (PubMed:18650437).

Biological Process

[2Fe-2S] cluster assemblyIEA:InterPro
Iron incorporation into metallo-sulfur clusterManual Assertion Based On ExperimentIDA:HGNC-UCL
Iron-sulfur cluster assemblyManual Assertion Based On ExperimentIBA:GO_Central
Mo-molybdopterin cofactor biosynthetic processIEA:UniProtKB-KW

Cellular Location

Isoform Mitochondrial
Mitochondrion
Isoform Cytoplasmic
Cytoplasm
Nucleus

Involvement in disease

Combined oxidative phosphorylation deficiency 52 (COXPD52):
An autosomal recessive mitochondrial disorder with onset in infancy, characterized by lactic acidemia, hypotonia, respiratory chain complex II and III deficiency, multisystem organ failure and abnormal mitochondria.

Sewell, K. E., Gola, G. F., Pignataro, M. F., Herrera, M. G., Noguera, M. E., Olmos, J., ... & Santos, J. (2023). Direct cysteine desulfurase activity determination by NMR and the study of the functional role of key structural elements of human NFS1. ACS Chemical Biology, 18(7), 1534-1547.

Uzarska, M. A., Grochowina, I., Soldek, J., Jelen, M., Schilke, B., Marszalek, J., ... & Dutkiewicz, R. (2022). During FeS cluster biogenesis, ferredoxin and frataxin use overlapping binding sites on yeast cysteine desulfurase Nfs1. Journal of Biological Chemistry, 298(2).

Fujihara, K. M., Zhang, B. Z., Jackson, T. D., Ogunkola, M. O., Nijagal, B., Milne, J. V., ... & Clemons, N. J. (2022). Eprenetapopt triggers ferroptosis, inhibits NFS1 cysteine desulfurase, and synergizes with serine and glycine dietary restriction. Science Advances, 8(37), eabm9427.

Armas, A. M., Balparda, M., Turowski, V. R., Busi, M. V., Pagani, M. A., & Gomez-Casati, D. F. (2019). Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants. Plant cell reports, 38, 981-990.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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