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Mouse Anti-NHLH2 Recombinant Antibody (2A3) (CBMAB-N2434-WJ)

This product is a Mouse antibody that recognizes NHLH2. The antibody 2A3 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all NHLH2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A3
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nescient Helix-Loop-Helix 2
Introduction
May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.
Entrez Gene ID
4808
UniProt ID
Q02577
Alternative Names
Nescient Helix-Loop-Helix 2; Class A Basic Helix-Loop-Helix Protein 34; Nescient Helix Loop Helix 2; BHLHa34; NSCL-2; HEN-2;
Function
Transcription factor which binds the E box motif 5'-CA[TC][AG]TG-3'. Involved in regulating energy expenditure, body mass, voluntary physical activity, mating behavior and reproductive longevity, acting through the hypothalamic-pituitary-gonadal axis. Acts as a transcriptional activator of target genes, including NDN and PCSK1. May act centrally to regulate function of both white and brown adipose tissue. Together with NHLH1, required to maintain migration and survival of cells in the anterior extramural migration stream (aes), which forms the precerebellar nuclei. Also, in concert with NHLH1, may determine fate of gonadotropin releasing hormone-1 (GnRH-1) neurons.
Biological Process
Apoptotic processIEA:Ensembl
Cell migration in hindbrainIEA:Ensembl
Central nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Hypothalamus gonadotrophin-releasing hormone neuron developmentIEA:Ensembl
Male gonad developmentIEA:Ensembl
Male mating behaviorIEA:Ensembl
Ovulation cycleIEA:Ensembl
Peripheral nervous system developmentIEA:Ensembl
Positive regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus

Madsen, A. T., & Good, D. J. (2023). In Silico Examination of Single Nucleotide Missense Mutations in NHLH2, a Gene Linked to Infertility and Obesity. International Journal of Molecular Sciences, 24(4), 3193.

Topaloglu, A. K., Simsek, E., Kocher, M. A., Mammadova, J., Bober, E., Kotan, L. D., ... & Good, D. J. (2022). Inactivating NHLH2 variants cause idiopathic hypogonadotropic hypogonadism and obesity in humans. Human Genetics, 141(2), 295-304.

Kummerfeld, D. M., Skryabin, B. V., Brosius, J., Vakhrushev, S. Y., & Rozhdestvensky, T. S. (2022). Reference genes across nine brain areas of wild type and Prader-Willi Syndrome mice: Assessing differences in Igfbp7, Pcsk1, Nhlh2 and Nlgn3 expression. International Journal of Molecular Sciences, 23(15), 8729.

Katzendorn, O., Peters, I., Dubrowinskaja, N., Moog, J. M., Reese, C., Tezval, H., ... & Serth, J. (2021). DNA methylation in INA, NHLH2, and THBS4 is associated with metastatic disease in renal cell carcinoma. Cancers, 14(1), 39.

Carraro, R. S., Nogueira, G. A., Sidarta-Oliveira, D., Gaspar, R. S., Dragano, N. R., Morari, J., ... & Velloso, L. A. (2021). Arcuate nucleus overexpression of NHLH2 reduces body mass and attenuates obesity-associated anxiety/depression-like behavior. Journal of Neuroscience, 41(48), 10004-10022.

Leon, S., Talbi, R., McCarthy, E. A., Ferrari, K., Fergani, C., Naule, L., ... & Navarro, V. M. (2021). Sex-specific pubertal and metabolic regulation of Kiss1 neurons via Nhlh2. Elife, 10, e69765.

Kocher, M. A., Huang, F. W., Le, E., & Good, D. J. (2021). Snord116 post-transcriptionally increases Nhlh2 mRNA stability: implications for human Prader-Willi syndrome. Human Molecular Genetics, 30(12), 1101-1110.

Hadziselimovic, F., Verkauskas, G., & Stadler, M. B. (2021). Molecular clues in the regulation of mini‐puberty involve neuronal DNA binding transcription factor NHLH2. Basic and clinical andrology, 31, 1-13.

Afshar, H., Adelirad, F., Kowsari, A., Kalhor, N., Delbari, A., Najafipour, R., ... & Ohadi, M. (2020). Natural selection at the NHLH2 core promoter exceptionally long CA-repeat in human and disease-only genotypes in late-onset neurocognitive disorder. Gerontology, 66(5), 514-522.

Li, W., Guo, B., Tao, K., Li, F., Liu, Z., Yao, H., ... & Liu, X. (2019). Inhibition of SIRT1 in hippocampal CA1 ameliorates PTSD-like behaviors in mice by protections of neuronal plasticity and serotonin homeostasis via NHLH2/MAO-A pathway. Biochemical and Biophysical Research Communications, 518(2), 344-350.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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