Mouse Anti-NHLH2 Recombinant Antibody (2A3) (CBMAB-N2434-WJ)

Name: Mouse Anti-NHLH2 Recombinant Antibody (2A3)
SKU: CBMAB-N2434-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A3

Application

ELISA, IF, WB

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nescient Helix-Loop-Helix 2

Introduction

May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.

Entrez Gene ID

4808

UniProt ID

Q02577

Alternative Names

Nescient Helix-Loop-Helix 2; Class A Basic Helix-Loop-Helix Protein 34; Nescient Helix Loop Helix 2; BHLHa34; NSCL-2; HEN-2;

Function

Transcription factor which binds the E box motif 5'-CA[TC][AG]TG-3'. Involved in regulating energy expenditure, body mass, voluntary physical activity, mating behavior and reproductive longevity, acting through the hypothalamic-pituitary-gonadal axis. Acts as a transcriptional activator of target genes, including NDN and PCSK1. May act centrally to regulate function of both white and brown adipose tissue. Together with NHLH1, required to maintain migration and survival of cells in the anterior extramural migration stream (aes), which forms the precerebellar nuclei. Also, in concert with NHLH1, may determine fate of gonadotropin releasing hormone-1 (GnRH-1) neurons.

Biological Process

Apoptotic processIEA:Ensembl
Cell migration in hindbrainIEA:Ensembl
Central nervous system developmentManual Assertion Based On ExperimentTAS:ProtInc
Hypothalamus gonadotrophin-releasing hormone neuron developmentIEA:Ensembl
Male gonad developmentIEA:Ensembl
Male mating behaviorIEA:Ensembl
Ovulation cycleIEA:Ensembl
Peripheral nervous system developmentIEA:Ensembl
Positive regulation of DNA-binding transcription factor activityManual Assertion Based On ExperimentTAS:BHF-UCL
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Madsen, A. T., & Good, D. J. (2023). In Silico Examination of Single Nucleotide Missense Mutations in NHLH2, a Gene Linked to Infertility and Obesity. International Journal of Molecular Sciences, 24(4), 3193.

Topaloglu, A. K., Simsek, E., Kocher, M. A., Mammadova, J., Bober, E., Kotan, L. D., ... & Good, D. J. (2022). Inactivating NHLH2 variants cause idiopathic hypogonadotropic hypogonadism and obesity in humans. Human Genetics, 141(2), 295-304.

Kummerfeld, D. M., Skryabin, B. V., Brosius, J., Vakhrushev, S. Y., & Rozhdestvensky, T. S. (2022). Reference genes across nine brain areas of wild type and Prader-Willi Syndrome mice: Assessing differences in Igfbp7, Pcsk1, Nhlh2 and Nlgn3 expression. International Journal of Molecular Sciences, 23(15), 8729.

Katzendorn, O., Peters, I., Dubrowinskaja, N., Moog, J. M., Reese, C., Tezval, H., ... & Serth, J. (2021). DNA methylation in INA, NHLH2, and THBS4 is associated with metastatic disease in renal cell carcinoma. Cancers, 14(1), 39.

Carraro, R. S., Nogueira, G. A., Sidarta-Oliveira, D., Gaspar, R. S., Dragano, N. R., Morari, J., ... & Velloso, L. A. (2021). Arcuate nucleus overexpression of NHLH2 reduces body mass and attenuates obesity-associated anxiety/depression-like behavior. Journal of Neuroscience, 41(48), 10004-10022.

Leon, S., Talbi, R., McCarthy, E. A., Ferrari, K., Fergani, C., Naule, L., ... & Navarro, V. M. (2021). Sex-specific pubertal and metabolic regulation of Kiss1 neurons via Nhlh2. Elife, 10, e69765.

Kocher, M. A., Huang, F. W., Le, E., & Good, D. J. (2021). Snord116 post-transcriptionally increases Nhlh2 mRNA stability: implications for human Prader-Willi syndrome. Human Molecular Genetics, 30(12), 1101-1110.

Hadziselimovic, F., Verkauskas, G., & Stadler, M. B. (2021). Molecular clues in the regulation of mini‐puberty involve neuronal DNA binding transcription factor NHLH2. Basic and clinical andrology, 31, 1-13.

Afshar, H., Adelirad, F., Kowsari, A., Kalhor, N., Delbari, A., Najafipour, R., ... & Ohadi, M. (2020). Natural selection at the NHLH2 core promoter exceptionally long CA-repeat in human and disease-only genotypes in late-onset neurocognitive disorder. Gerontology, 66(5), 514-522.

Li, W., Guo, B., Tao, K., Li, F., Liu, Z., Yao, H., ... & Liu, X. (2019). Inhibition of SIRT1 in hippocampal CA1 ameliorates PTSD-like behaviors in mice by protections of neuronal plasticity and serotonin homeostasis via NHLH2/MAO-A pathway. Biochemical and Biophysical Research Communications, 518(2), 344-350.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NHLH2 Matched Antibody Pair (799) (CAT#: APMAB-799LY)

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Mouse Anti-NHLH2 Recombinant Antibody (3E3) (CAT#: CBMAB-N2430-WJ)

NHLH2 Matched Antibody Pair (799) (CAT#: APMAB-799LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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