Mouse Anti-NID1 Recombinant Antibody (2R33) (CBMAB-N2449-WJ)

Name: Mouse Anti-NID1 Recombinant Antibody (2R33)
SKU: CBMAB-N2449-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2R33

Application

WB, ELISA, IHC, IHC-P

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nidogen 1

Introduction

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4811

UniProt ID

P14543

Alternative Names

Nidogen 1; Entactin; NID-1; NID; Nidogen (Enactin); Nidogen-1; Enactin;

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Biological Process

Basement membrane organizationManual Assertion Based On ExperimentTAS:BHF-UCL
Cell-matrix adhesionIEA:Ensembl
Glomerular basement membrane developmentIEA:Ensembl
Positive regulation of cell adhesion1 PublicationIC:ComplexPortal
Positive regulation of cell-substrate adhesionIEA:Ensembl
Positive regulation of integrin-mediated signaling pathway1 PublicationIC:ComplexPortal
Positive regulation of muscle cell differentiation1 PublicationIC:ComplexPortal
Regulation of basement membrane organization1 PublicationIC:ComplexPortal

Cellular Location

Secreted, extracellular space, extracellular matrix, basement membrane

PTM

N- and O-glycosylated.

Rokavec, M., Jaeckel, S., & Hermeking, H. (2023). Nidogen-1/NID1 Function and Regulation during Progression and Metastasis of Colorectal Cancer. Cancers, 15(22), 5316.

Ijezie, E. C., O'Dowd, J. M., Kuan, M. I., Faeth, A. R., & Fortunato, E. A. (2023). HCMV Infection Reduces Nidogen-1 Expression, Contributing to Impaired Neural Rosette Development in Brain Organoids. Journal of Virology, 97(5), e01718-22.

Bayramoglu, Z., Kılınc, A. N., Omeroglu, E., Yilmaz, F., Bayramoglu, D., Unlu, Y., & Aydin, H. A. (2022). Expression of extracellular matrix proteins nidogen‐1 and legumain in endometrial carcinomas. Journal of Obstetrics and Gynaecology Research, 48(4), 1019-1025.

Zbinden, A., Layland, S. L., Urbanczyk, M., Carvajal Berrio, D. A., Marzi, J., Zauner, M., ... & Schenke‐Layland, K. (2021). Nidogen‐1 mitigates ischemia and promotes tissue survival and regeneration. Advanced Science, 8(4), 2002500.

Jagroop, R., Martin, C. J., & Moorehead, R. A. (2021). Nidogen 1 regulates proliferation and migration/invasion in murine claudin‑low mammary tumor cells. Oncology Letters, 21(1), 1-1.

Zhang, B., Xu, C., Liu, J., Yang, J., Gao, Q., & Ye, F. (2021). Nidogen-1 expression is associated with overall survival and temozolomide sensitivity in low-grade glioma patients. Aging (Albany NY), 13(6), 9085.

Kuan, M. I., Jaeger, H. K., Balemba, O. B., O’Dowd, J. M., Duricka, D., Hannemann, H., ... & Fortunato, E. A. (2021). Human cytomegalovirus interactions with the basement membrane protein nidogen 1. Journal of Virology, 95(3), e01506-20.

Khaerunnisa, S., Pangjaya, L. F., Mazfufah, N. F., Budiman, R. L., & Antarianto, R. D. (2021, March). Nidogen-1 (NID1) as specific ligand in natural killer (NK) cells stimulated from advanced ovarian cancer: Immunotherapy review. In AIP Conference Proceedings (Vol. 2344, No. 1). AIP Publishing.

Jagroop, R. (2019). The Effects of Nidogen-1 on Claudin-Low Mammary Tumor Cells (Doctoral dissertation, University of Guelph).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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