Mouse Anti-NIT2 Recombinant Antibody (6H7) (CBMAB-N2495-WJ)

Name: Mouse Anti-NIT2 Recombinant Antibody (6H7)
SKU: CBMAB-N2495-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6H7

Application

WB, IHC, IF

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

0.71 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nitrilase family, member 2

Introduction

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Entrez Gene ID

56954

UniProt ID

Q9NQR4

Alternative Names

Nitrilase Family Member 2; Nitrilase Homolog 2; Omega-Amidase NIT2; Epididymis Secretory Sperm Binding Protein Li 8a; Nitrilase Family, Member 2; Nit Protein 2; EC 3.5.1.3; HEL-S-8a;

Function

Has omega-amidase activity (PubMed:22674578, PubMed:19595734).
The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively (PubMed:19595734).

Biological Process

Asparagine metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Glutamine metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Oxaloacetate metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Cytoplasm

Zhang, H., Zhang, H., Qin, X., Wang, X., Wang, Y., Tu, T., ... & Luo, H. (2022). Biodegradation of nitriles derived from glucosinolates in rapeseed meal by Bn NIT2: a nitrilase from Brassica napus with wide substrate specificity. Applied Microbiology and Biotechnology, 106(7), 2445-2454.

Yang, S., Zhang, T., Wang, Z., Zhao, X., Li, R., & Li, J. (2022). Nitrilases NIT1/2/3 positively regulate flowering by Inhibiting MAF4 expression in Arabidopsis. Frontiers in Plant Science, 13, 889460.

Silva Teixeira, C. S., Sousa, S. F., & Cerqueira, N. M. (2021). An Unsual Cys‐Glu‐Lys Catalytic Triad is Responsible for the Catalytic Mechanism of the Nitrilase Superfamily: A QM/MM Study on Nit2. ChemPhysChem, 22(8), 796-804.

Jin, C., Jin, H., Jeong, B. C., Cho, D. H., Chun, H. S., Kim, W. K., & Chang, J. H. (2021). Crystal Structure of Nitrilase-Like Protein Nit2 from Kluyveromyces lactis. Crystals, 11(5), 499.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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