NIT2

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Full Name

nitrilase family, member 2

Function

Has omega-amidase activity (PubMed:22674578, PubMed:19595734).
The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively (PubMed:19595734).

Biological Process

Asparagine metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Glutamine metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Oxaloacetate metabolic processManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Cytoplasm

Anti-NIT2 antibodies

Mouse Anti-NIT2 Recombinant Antibody (6H7) (CBMAB-N2495-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: 6H7

Application*: WB, IH, IF

Mouse Anti-NIT2 Recombinant Antibody (5A3) (CBMAB-N2494-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 5A3

Application*: WB, IH, IF

Mouse Anti-NIT2 Recombinant Antibody (3F3) (CBMAB-N2493-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: 3F3

Application*: IF, IH, WB

Mouse Anti-NIT2 Recombinant Antibody (3D5) (CBMAB-N2492-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: 3D5

Application*: F, IF, P, WB

Mouse Anti-NIT2 Recombinant Antibody (2B9) (CBMAB-N2491-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: 2B9

Application*: WB, F, IH, IF, P

Mouse Anti-NIT2 Recombinant Antibody (1A6) (CBMAB-N2490-WJ)

Datasheet

SDS

Target: NIT2

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 1A6

Application*: IH, P, WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)