Mouse Anti-NKX6-1 Recombinant Antibody (11D12) (CBMAB-N2632-WJ)

Name: Mouse Anti-NKX6-1 Recombinant Antibody (11D12)
SKU: CBMAB-N2632-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

11D12

Application

ELISA, ICC, WB

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, 5% Trehalose

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Nkx6-1

Introduction

In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008]

Entrez Gene ID

4825

UniProt ID

P78426

Alternative Names

NK6 Homeobox 1; Homeobox Protein NK-6 Homolog A; NKX6A; NK6 Transcription Factor Related, Locus 1 (Drosophila); NK6 Transcription Factor Related, Locus 1; NK Homeobox (Drosophila), Family 6, A;

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity).
Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).

Biological Process

Animal organ morphogenesisManual Assertion Based On ExperimentTAS:ProtInc
Cell differentiationManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to cytokine stimulusIEA:Ensembl
Cellular response to peptide hormone stimulusIEA:Ensembl
Central nervous system neuron differentiationIEA:Ensembl
Detection of glucoseISS:BHF-UCL
Negative regulation of glial cell differentiationIEA:Ensembl
Negative regulation of transcription by RNA polymerase IIISS:BHF-UCL
Neuron migrationIEA:InterPro
Oligodendrocyte differentiationIEA:Ensembl
Pancreas developmentISS:BHF-UCL
Positive regulation of DNA-binding transcription factor activityISS:BHF-UCL
Positive regulation of glial cell differentiationIEA:Ensembl
Positive regulation of insulin secretionIEA:Ensembl
Positive regulation of neuron differentiationIEA:Ensembl
Positive regulation of transcription by RNA polymerase IIISS:BHF-UCL
Positive regulation of type B pancreatic cell developmentISS:BHF-UCL
Regulation of axon extensionIEA:Ensembl
Regulation of neuron migrationIEA:Ensembl
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Response to nicotineIEA:Ensembl
Response to xenobiotic stimulusIEA:Ensembl
Smoothened signaling pathwayIEA:Ensembl
Transcription by RNA polymerase IIIEA:Ensembl
Type B pancreatic cell maturationISS:BHF-UCL
Type B pancreatic cell proliferationISS:UniProtKB

Cellular Location

Nucleus

Pavluch, V., Engstová, H., Špačková, J., & Ježek, P. (2023). Deficiency of transcription factor Nkx6. 1 does not prevent insulin secretion in INS-1E cells. Scientific Reports, 13(1), 683.

Xie, B., Tong, K., Yang, J., Wang, T., Cheng, L., Zeng, S., & Hu, Z. (2022). NKX6-1 is a less sensitive but specific biomarker of chromophobe renal cell carcinoma. The American Journal of Surgical Pathology, 46(6), 809-815.

Patel, M., Anderson, J., Lei, S., Finkel, Z., Rodriguez, B., Esteban, F., ... & Cai, L. (2021). Nkx6. 1 enhances neural stem cell activation and attenuates glial scar formation and neuroinflammation in the adult injured spinal cord. Experimental neurology, 345, 113826.

Fujita, Y., Kozawa, J., Fukui, K., Iwahashi, H., Eguchi, H., & Shimomura, I. (2021). Increased NKX6. 1 expression and decreased ARX expression in alpha cells accompany reduced beta-cell volume in human subjects. Scientific Reports, 11(1), 17796.

Memon, B., Younis, I., Abubaker, F., & Abdelalim, E. M. (2021). PDX1−/NKX6. 1+ progenitors derived from human pluripotent stem cells as a novel source of insulin‐secreting cells. Diabetes/Metabolism Research and Reviews, 37(5), e3400.

Zhang, F. F., Liu, Y. H., Wang, D. W., Liu, T. S., Yang, Y., Guo, J. M., ... & Jin, L. (2020). Obesity-induced reduced expression of the lncRNA ROIT impairs insulin transcription by downregulation of Nkx6. 1 methylation. Diabetologia, 63, 811-824.

Aigha, I. I., & Abdelalim, E. M. (2020). NKX6. 1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation. Stem Cell Research & Therapy, 11(1), 1-14.

Chung, H. H., Lee, C. T., Hu, J. M., Chou, Y. C., Lin, Y. W., & Shih, Y. L. (2020). NKX6. 1 represses tumorigenesis, metastasis, and chemoresistance in colorectal cancer. International Journal of Molecular Sciences, 21(14), 5106.

Tran, R., Moraes, C., & Hoesli, C. A. (2020). Controlled clustering enhances PDX1 and NKX6. 1 expression in pancreatic endoderm cells derived from pluripotent stem cells. Scientific reports, 10(1), 1190.

Padula, S. L., Anand, D., Hoang, T. V., Chaffee, B. R., Liu, L., Liang, C., ... & Robinson, M. L. (2019). High-throughput transcriptome analysis reveals that the loss of Pten activates a novel NKX6-1/RASGRP1 regulatory module to rescue microphthalmia caused by Fgfr2-deficient lenses. Human genetics, 138, 1391-1407.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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