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Rabbit Anti-NLK Recombinant Antibody (D9X3C) (CBMAB-CP1724-LY)

The product is antibody recognizes NLK. The antibody D9X3C immunoassay techniques such as: WB.
See all NLK antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D9X3C
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NLK protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Nemo Like Kinase
Introduction
Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
Entrez Gene ID
Human51701
Mouse18099
Rat497961
UniProt ID
HumanQ9UBE8
MouseO54949
RatD3ZSZ3
Alternative Names
Nemo Like Kinase; Nemo-Like Kinase; EC 2.7.11.24; Protein LAK1; EC 2.7.11; LAK1;
Function
Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
Biological Process
Intracellular signal transductionISS:UniProtKB
Negative regulation of Wnt signaling pathwayISS:UniProtKB
Peptidyl-threonine phosphorylationISS:UniProtKB
Protein autophosphorylationIEA:Ensembl
Protein phosphorylationISS:UniProtKB
Protein stabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription, DNA-templatedISS:UniProtKB
Serine phosphorylation of STAT proteinISS:UniProtKB
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Wnt signaling pathway, calcium modulating pathwayTAS:Reactome
Cellular Location
Nucleus
Cytoplasm
Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
PTM
Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

Yin, X., Ren, Y., Luo, W., Liao, M., Huang, L., Zhuang, X., ... & Wang, W. (2022). Nemo-like kinase (NLK) gene regulates apoptosis via the p53 signaling pathway in Litopenaeus vannamei under low-temperature stress. Developmental & Comparative Immunology, 131, 104378.

Yang, B., Chen, S., & Zang, Y. (2022). The Mechanism of Nemo-Like Kinase (NLK) in Non-Small Cell Lung Cancer (NSCLC) Cells by Regulating Vascular Endothelial Growth Factor (VEGF). Journal of Biomaterials and Tissue Engineering, 12(12), 2352-2357.

Ji, Y. X., Wang, Y., Li, P. L., Cai, L., Wang, X. M., Bai, L., ... & Li, H. (2021). A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis. Cell Metabolism, 33(6), 1171-1186.

Liang, J., Zhou, Y., Zhang, N., Wang, D., Cheng, X., Li, K., ... & Song, W. (2021). The phosphorylation of the Smad2/3 linker region by nemo-like kinase regulates TGF-β signaling. Journal of Biological Chemistry, 296.

Daams, R., & Massoumi, R. (2020). Nemo-like kinase in development and diseases: insights from mouse studies. International Journal of Molecular Sciences, 21(23), 9203.

Jiang, M., Zhang, X., Liu, H., LeBron, J., Alexandris, A., Peng, Q., ... & Duan, W. (2020). Nemo-like kinase reduces mutant huntingtin levels and mitigates Huntington’s disease. Human molecular genetics, 29(8), 1340-1352.

Daams, R., Sime, W., Leandersson, K., Sitnicka, E., & Massoumi, R. (2020). Deletion of nemo-like kinase in T cells reduces single-positive CD8+ thymocyte population. The Journal of Immunology, 205(7), 1830-1841.

Lei, L., Wang, Y., Zheng, Y. W., Fei, L. R., Shen, H. Y., Li, Z. H., ... & Xu, H. T. (2019). Overexpression of Nemo-like kinase promotes the proliferation and invasion of lung cancer cells and indicates poor prognosis. Current Cancer Drug Targets, 19(8), 674-680.

Li, S. Z., Shu, Q. P., Song, Y., Zhang, H. H., Liu, Y., Jin, B. X., ... & Zhang, X. D. (2019). Phosphorylation of MAVS/VISA by Nemo-like kinase (NLK) for degradation regulates the antiviral innate immune response. Nature Communications, 10(1), 3233.

Shi, C., Xu, L., Tang, Z., Zhang, W., Wei, Y., Ni, J., ... & Feng, J. (2019). Knockdown of Nemo‑like kinase promotes metastasis in non‑small‑cell lung cancer. Oncology Reports, 42(3), 1090-1100.

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For research use only. Not intended for any clinical use.

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