Human Recombinant NLK protein, GST Tag (V2LY-0526-LY5809)

Name: Human Recombinant NLK protein, GST Tag
SKU: V2LY-0526-LY5809
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant NLK protein, GST Tag consist of Amino Acid: 121-527 and predicts a molecular mass of 74.1 kDa.

Molecule Mass

74.1 kDa

Sequence

Amino Acid: 121-527

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>91% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Nemo Like Kinase

Function

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).

Biological Process

Intracellular signal transductionISS:UniProtKB
Negative regulation of Wnt signaling pathwayISS:UniProtKB
Peptidyl-threonine phosphorylationISS:UniProtKB
Protein autophosphorylationIEA:Ensembl
Protein phosphorylationISS:UniProtKB
Protein stabilizationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription, DNA-templatedISS:UniProtKB
Serine phosphorylation of STAT proteinISS:UniProtKB
Transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Wnt signaling pathway, calcium modulating pathwayTAS:Reactome

Cellular Location

Nucleus
Cytoplasm
Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).

PTM

Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

Yin, X., Ren, Y., Luo, W., Liao, M., Huang, L., Zhuang, X., ... & Wang, W. (2022). Nemo-like kinase (NLK) gene regulates apoptosis via the p53 signaling pathway in Litopenaeus vannamei under low-temperature stress. Developmental & Comparative Immunology, 131, 104378.

Yang, B., Chen, S., & Zang, Y. (2022). The Mechanism of Nemo-Like Kinase (NLK) in Non-Small Cell Lung Cancer (NSCLC) Cells by Regulating Vascular Endothelial Growth Factor (VEGF). Journal of Biomaterials and Tissue Engineering, 12(12), 2352-2357.

Ji, Y. X., Wang, Y., Li, P. L., Cai, L., Wang, X. M., Bai, L., ... & Li, H. (2021). A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis. Cell Metabolism, 33(6), 1171-1186.

Liang, J., Zhou, Y., Zhang, N., Wang, D., Cheng, X., Li, K., ... & Song, W. (2021). The phosphorylation of the Smad2/3 linker region by nemo-like kinase regulates TGF-β signaling. Journal of Biological Chemistry, 296.

Daams, R., & Massoumi, R. (2020). Nemo-like kinase in development and diseases: insights from mouse studies. International Journal of Molecular Sciences, 21(23), 9203.

Jiang, M., Zhang, X., Liu, H., LeBron, J., Alexandris, A., Peng, Q., ... & Duan, W. (2020). Nemo-like kinase reduces mutant huntingtin levels and mitigates Huntington’s disease. Human molecular genetics, 29(8), 1340-1352.

Daams, R., Sime, W., Leandersson, K., Sitnicka, E., & Massoumi, R. (2020). Deletion of nemo-like kinase in T cells reduces single-positive CD8+ thymocyte population. The Journal of Immunology, 205(7), 1830-1841.

Lei, L., Wang, Y., Zheng, Y. W., Fei, L. R., Shen, H. Y., Li, Z. H., ... & Xu, H. T. (2019). Overexpression of Nemo-like kinase promotes the proliferation and invasion of lung cancer cells and indicates poor prognosis. Current Cancer Drug Targets, 19(8), 674-680.

Li, S. Z., Shu, Q. P., Song, Y., Zhang, H. H., Liu, Y., Jin, B. X., ... & Zhang, X. D. (2019). Phosphorylation of MAVS/VISA by Nemo-like kinase (NLK) for degradation regulates the antiviral innate immune response. Nature Communications, 10(1), 3233.

Shi, C., Xu, L., Tang, Z., Zhang, W., Wei, Y., Ni, J., ... & Feng, J. (2019). Knockdown of Nemo‑like kinase promotes metastasis in non‑small‑cell lung cancer. Oncology Reports, 42(3), 1090-1100.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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Alternative Versions

Human Recombinant NLK, Active protein, GST Tag (CAT#: V2LY-0526-LY5808)

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Isotype control

For research use only. Not intended for any clinical use.

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