Rat Anti-NPR1 Recombinant Antibody (CBWJN-0542) (CBMAB-N3240-WJ)

Name: Rat Anti-NPR1 Recombinant Antibody (CBWJN-0542)
SKU: CBMAB-N3240-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBWJN-0542

Application

ELISA, IHC

Specificity

Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, pH 7.4, 5% Trehalose

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NATRIURETIC PEPTIDE RECEPTOR 1

Introduction

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]

Entrez Gene ID

18160

UniProt ID

P18293

Alternative Names

Natriuretic Peptide Receptor 1; Guanylate Cyclase A; Atrial Natriuretic Peptide Receptor Type A; Atrionatriuretic Peptide Receptor A; Natriuretic Peptide Receptor A; EC 4.6.1.2; ANPR-A; ANPRA; ANP-A; NPR-A;

Function

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Biological Process

Blood vessel diameter maintenance1 PublicationNAS:UniProtKB
Body fluid secretionManual Assertion Based On ExperimentTAS:UniProtKB
Cell surface receptor signaling pathway1 PublicationNAS:UniProtKB
cGMP biosynthetic processManual Assertion Based On ExperimentIDA:GO_Central
cGMP-mediated signalingIEA:Ensembl
Dopamine metabolic processIEA:Ensembl
Negative regulation of angiogenesisManual Assertion Based On ExperimentTAS:UniProtKB
Negative regulation of cell growth1 PublicationNAS:UniProtKB
Negative regulation of smooth muscle cell proliferationIEA:Ensembl
Positive regulation of cGMP-mediated signalingIEA:Ensembl
Positive regulation of renal sodium excretionManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of urine volumeManual Assertion Based On ExperimentTAS:UniProtKB
Receptor guanylyl cyclase signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of blood pressure1 PublicationNAS:UniProtKB
Regulation of vascular permeabilityManual Assertion Based On ExperimentTAS:UniProtKB
Signal transductionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Membrane

Topology

Extracellular: 33-473
Helical: 474-494
Cytoplasmic: 495-1061

PTM

Phosphorylation of the protein kinase-like domain is required for full activation by ANP.

Sasahara, M., Kanda, M., Tanaka, C., Shimizu, D., Umeda, S., Takami, H., ... & Kodera, Y. (2024). Therapeutic antibody targeting natriuretic peptide receptor 1 inhibits gastric cancer growth via BCL‐2‐mediated intrinsic apoptosis. International Journal of Cancer, 154(7), 1272-1284.

Tokudome, T., Otani, K., Mao, Y., Jensen, L. J., Arai, Y., Miyazaki, T., ... & Nishimura, H. (2022). Endothelial natriuretic peptide receptor 1 play crucial role for acute and chronic blood pressure regulation by atrial natriuretic peptide. Hypertension, 79(7), 1409-1422.

Pandey, K. N. (2019). Genetic ablation and guanylyl cyclase/natriuretic peptide receptor-A: impact on the pathophysiology of cardiovascular dysfunction. International Journal of Molecular Sciences, 20(16), 3946.

Solinski, H. J., Dranchak, P., Oliphant, E., Gu, X., Earnest, T. W., Braisted, J., ... & Hoon, M. A. (2019). Inhibition of natriuretic peptide receptor 1 reduces itch in mice. Science translational medicine, 11(500), eaav5464.

Periyasamy, R., Das, S., & Pandey, K. N. (2019). Genetic disruption of guanylyl cyclase/natriuretic peptide receptor-A upregulates renal (pro) renin receptor expression in Npr1 null mutant mice. Peptides, 114, 17-28.

Vandenwijngaert, S., Ledsky, C. D., Lahrouchi, N., Khan, M. A., Wunderer, F., Ames, L., ... & CHARGE+ Exome Chip Blood Pressure Consortium, the CHD Exome+ Consortium, the Exome BP Consortium, the GoT2D Consortium, the T2D-GENES Consortium and the UK Biobank CardioMetabolic Consortium BP Working Group. (2019). Blood pressure-associated genetic variants in the natriuretic peptide receptor 1 gene modulate guanylate cyclase activity. Circulation: Genomic and Precision Medicine, 12(8), e002472.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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