Rabbit Anti-NQO2 Recombinant Antibody (
8E12) (V2LY-0725-LY1445)

Name: Rabbit Anti-NQO2 Recombinant Antibody (8E12)
SKU: V2LY-0725-LY1445
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-NQO2 Recombinant Antibody (8E12) (V2LY-0725-LY1445) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Mouse liver tissue
All lanes: NQO2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

8E12

Application

ELISA, WB

Immunogen

A synthesized peptide derived from human NQO2.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

N-Ribosyldihydronicotinamide:Quinone Reductase 2

Entrez Gene ID

4835

UniProt ID

P16083

Function

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Biological Process

Quinone catabolic processManual Assertion Based On ExperimentIDA:CAFA

Cellular Location

Cytoplasm

Janda, E., Boutin, J. A., De Lorenzo, C., & Arbitrio, M. (2024). Polymorphisms and Pharmacogenomics of NQO2: The Past and the Future. Genes, 15(1), 87.

Rashid, M. H., Babu, D., & Siraki, A. G. (2021). Interactions of the antioxidant enzymes NAD (P) H: Quinone oxidoreductase 1 (NQO1) and NRH: Quinone oxidoreductase 2 (NQO2) with pharmacological agents, endogenous biochemicals and environmental contaminants. Chemico-Biological Interactions, 345, 109574.

Schepers, A. G., Shan, J., Cox, A. G., Huang, A., Evans, H., Walesky, C., ... & Bhatia, S. N. (2021). Identification of NQO2 As a Protein Target in Small Molecule Modulation of Hepatocellular Function. ACS Chemical Biology, 16(9), 1770-1778.

Janda, E., Nepveu, F., Calamini, B., Ferry, G., & Boutin, J. A. (2020). Molecular pharmacology of NRH: quinone oxidoreductase 2: A detoxifying enzyme acting as an undercover toxifying enzyme. Molecular Pharmacology, 98(5), 620-633.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

NQO2 Matched Antibody Pair (815) (CAT#: APMAB-815LY)

NQO2 Matched Antibody Pair (816) (CAT#: APMAB-816LY)

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Mouse Anti-NQO2 Recombinant Antibody (5F6) (CAT#: CBMAB-N3313-WJ)

NQO2 Matched Antibody Pair (815) (CAT#: APMAB-815LY)

Mouse Anti-NQO2 Recombinant Antibody (CBWJN-0766) (CAT#: CBMAB-N3316-WJ)

Mouse Anti-NQO2 Recombinant Antibody (3C11) (CAT#: CBMAB-N3308-WJ)

Mouse Anti-NQO2 Recombinant Antibody (11H4) (CAT#: CBMAB-N3305-WJ)

Mouse Anti-NQO2 Recombinant Antibody (3E8-G2) (CAT#: CBMAB-A6091-LY)

NQO2 Matched Antibody Pair (816) (CAT#: APMAB-816LY)

Mouse Anti-NQO2 Recombinant Antibody (AT1E3) (CAT#: CBMAB-N3314-WJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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