Mouse Anti-NSD3 Recombinant Antibody (1F11) (CBMAB-N0006-WJ)

This product is a mouse antibody that recognizes NSD3. The antibody 1F11 can be used for immunoassay techniques such as: WB.
See all NSD3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1F11

Antibody Isotype

IgG1

Application

Basic Information

Immunogen

Human recombinant protein fragment corresponding to aa427-566 of human WHSC1L1(NP_060248) produced in E.coli.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nuclear Receptor Binding SET Domain Protein 3

Introduction

NSD3 is a protein-coding gene which is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Diseases associated with NSD3 include Leukemia, Acute Myeloid and Wolf-Hirschhorn Syndrome. An important paralog of this gene is NSD2.

Entrez Gene ID

54904

UniProt ID

Q9BZ95

Alternative Names

KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328

Function

Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.

Biological Process

Chromatin organizationIEA:UniProtKB-KW
Histone methylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of histone H3-K36 trimethylationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Chromosome

Involvement in disease

Defects in NSD3 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
A chromosomal aberration involving NSD3 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.

References

Xiong, Q., Zhou, Y., Zhang, S., Zhang, Y., Xu, Y., Yang, Y., ... & Zhu, Q. (2023). NSD3, a member of nuclear receptor‐binding SET domain family, is a potential prognostic biomarker for pancreatic cancer. Cancer Medicine, 12(9), 10961-10978.

Kim, S., Hwang, I., Kim, S. H., Chung, H. W., Ji, M. J., Moon, S., ... & Hur, W. (2023). Identification of novel class inhibitors of NSD3 methyltransferase showing a unique, bivalent binding mode in the SET domain. Chemical Biology & Drug Design, 102(3), 500-513.

Topchu, I., Pangeni, R. P., Bychkov, I., Miller, S. A., Izumchenko, E., Yu, J., ... & Boumber, Y. (2022). The role of NSD1, NSD2, and NSD3 histone methyltransferases in solid tumors. Cellular and Molecular Life Sciences, 79(6), 285.

Sun, Y., Xie, J., Cai, S., Wang, Q., Feng, Z., Li, Y., ... & Ye, Z. (2021). Elevated expression of nuclear receptor-binding SET domain 3 promotes pancreatic cancer cell growth. Cell Death & Disease, 12(10), 913.

Rathert, P. (2021). Structure, activity and function of the NSD3 protein lysine methyltransferase. Life, 11(8), 726.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NSD3 Recombinant Antibody (8G3)

Rabbit Anti-NSD3 Recombinant Antibody (D4N9N)

Mouse Anti-NSD3 Recombinant Antibody (CBWJN-0705)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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