Rabbit Anti-NTMT1 Recombinant Antibody (D9D6P) (CBMAB-N3604-WJ)

Name: Rabbit Anti-NTMT1 Recombinant Antibody (D9D6P)
SKU: CBMAB-N3604-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D9D6P

Application

Specificity

Human, Mouse, Rat, Monkey

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

N-Terminal Xaa-Pro-Lys N-Methyltransferase 1

Introduction

NTMT1 (N-Terminal Xaa-Pro-Lys N-Methyltransferase 1) is a Protein Coding gene. Among its related pathways are DNA Damage. Gene Ontology (GO) annotations related to this gene include methyltransferase activity and O-methyltransferase activity. An important paralog of this gene is METTL11B.

Entrez Gene ID

Human	28989
Mouse	66617
Rat	362103
Monkey	716763

UniProt ID

Human	Q9BV86
Mouse	Q8R2U4
Rat	Q5BJX0
Monkey	F7HMB4

Alternative Names

AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NRMT1, NTM1A

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation (PubMed:24090352).
Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

Biological Process

Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Histone methylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-alanine methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-alanine trimethylationIEA:Ensembl
N-terminal peptidyl-glycine methylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-proline dimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-proline methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-serine dimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-serine methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-serine trimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal protein amino acid methylationManual Assertion Based On ExperimentIBA:GO_Central
Spindle organizationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus
Predominantly nuclear (PubMed:24090352).

Zhao, C., Yu, M., & Li, Y. (2024). Pan‐cancer analysis reveals the pro‐oncogenic role of N6‐methyladenosine (m6A)‐regulated NTMT1 in head and neck squamous cell carcinoma. Journal of Biochemical and Molecular Toxicology, 38(1), e23603.

Deng, Y., Dong, G., Meng, Y., Noinaj, N., & Huang, R. (2023). Structure–activity relationship studies of Venglustat on NTMT1 inhibition. Journal of Medicinal Chemistry, 66(2), 1601-1615.

Zhao, Y., Wang, Z., Yu, Q., Liu, X., Liu, X., Dong, S., ... & Wang, S. (2022). Robust performance of the methylated NTMT1 and MAP3K14-AS1 dual-target test for colorectal cancer detection in plasma by using sense-antisense and dual-MGB probe technique.

Chen, D., Meng, Y., Yu, D., Noinaj, N., Cheng, X., & Huang, R. (2021). Chemoproteomic study uncovers HemK2/KMT9 as a new target for NTMT1 bisubstrate inhibitors. ACS chemical biology, 16(7), 1234-1242.

Mackie, B. D., Chen, D., Dong, G., Dong, C., Parker, H., Schaner Tooley, C. E., ... & Huang, R. (2020). Selective peptidomimetic inhibitors of NTMT1/2: rational design, synthesis, characterization, and crystallographic studies. Journal of medicinal chemistry, 63(17), 9512-9522.

Jia, K., Huang, G., Wu, W., Shrestha, R., Wu, B., Xiong, Y., & Li, P. (2019). In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1. Chemical science, 10(35), 8094-8099.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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