Human Recombinant METTL11A protein, GST Tag-1 (V2LY-0526-LY5565)

Name: Human Recombinant METTL11A protein, GST Tag-1
SKU: V2LY-0526-LY5565
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant METTL11A protein, GST Tag consist of Amino Acid: 2-223 and predicts a molecular mass of 52.2 kDa.

Molecule Mass

52.2 kDa

Sequence

Amino Acid: 2-223

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥70% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MOPS, NaCl, Glutathione, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

N-Terminal Xaa-Pro-Lys N-Methyltransferase 1

Function

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by NTMT2-mediated monomethylation (PubMed:24090352).
Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.

Biological Process

Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Histone methylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-alanine methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-alanine trimethylationIEA:Ensembl
N-terminal peptidyl-glycine methylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-proline dimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-proline methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-serine dimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal peptidyl-serine methylationManual Assertion Based On ExperimentIBA:GO_Central
N-terminal peptidyl-serine trimethylationManual Assertion Based On ExperimentIDA:UniProtKB
N-terminal protein amino acid methylationManual Assertion Based On ExperimentIBA:GO_Central
Spindle organizationManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Nucleus
Predominantly nuclear (PubMed:24090352).

Zhao, C., Yu, M., & Li, Y. (2024). Pan‐cancer analysis reveals the pro‐oncogenic role of N6‐methyladenosine (m6A)‐regulated NTMT1 in head and neck squamous cell carcinoma. Journal of Biochemical and Molecular Toxicology, 38(1), e23603.

Deng, Y., Dong, G., Meng, Y., Noinaj, N., & Huang, R. (2023). Structure–activity relationship studies of Venglustat on NTMT1 inhibition. Journal of Medicinal Chemistry, 66(2), 1601-1615.

Zhao, Y., Wang, Z., Yu, Q., Liu, X., Liu, X., Dong, S., ... & Wang, S. (2022). Robust performance of the methylated NTMT1 and MAP3K14-AS1 dual-target test for colorectal cancer detection in plasma by using sense-antisense and dual-MGB probe technique.

Chen, D., Meng, Y., Yu, D., Noinaj, N., Cheng, X., & Huang, R. (2021). Chemoproteomic study uncovers HemK2/KMT9 as a new target for NTMT1 bisubstrate inhibitors. ACS chemical biology, 16(7), 1234-1242.

Mackie, B. D., Chen, D., Dong, G., Dong, C., Parker, H., Schaner Tooley, C. E., ... & Huang, R. (2020). Selective peptidomimetic inhibitors of NTMT1/2: rational design, synthesis, characterization, and crystallographic studies. Journal of medicinal chemistry, 63(17), 9512-9522.

Jia, K., Huang, G., Wu, W., Shrestha, R., Wu, B., Xiong, Y., & Li, P. (2019). In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1. Chemical science, 10(35), 8094-8099.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant METTL11A protein (CAT#: V2LY-0526-LY5563)

Human Recombinant METTL11A protein, GST Tag (CAT#: V2LY-0526-LY5564)

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Human Recombinant METTL11A protein, GST Tag (CAT#: V2LY-0526-LY5564)

Human Recombinant METTL11A protein (CAT#: V2LY-0526-LY5563)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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