Mouse Anti-NUDT12 Recombinant Antibody (3F7) (CBMAB-N3878-WJ)

Name: Mouse Anti-NUDT12 Recombinant Antibody (3F7)
SKU: CBMAB-N3878-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F7

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG3, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

nudix (nucleoside diphosphate linked moiety X)-type motif 12

Introduction

Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12, regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al., 2003 [PubMed 12790796]).[supplied by OMIM, Mar 2008]

Entrez Gene ID

83594

UniProt ID

Q9BQG2

Alternative Names

Nudix Hydrolase 12; EC 3.6.1.22; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 12; Nucleoside Diphosphate Linked Moiety X-Type Motif 12; Nucleoside Diphosphate-Linked Moiety X Motif 12; Nudix Motif 12;

Function

mRNA decapping enzyme that specifically removes the nicotinamide adenine dinucleotide (NAD) cap from a subset of mRNAs by hydrolyzing the diphosphate linkage to produce nicotinamide mononucleotide (NMN) and 5' monophosphate mRNA (PubMed:31101919, PubMed:31875550).
The NAD-cap is present at the 5'-end of some RNAs; in contrast to the canonical N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed:31101919).
Preferentially acts on NAD-capped transcripts in response to nutrient stress (PubMed:31101919).
Also acts on free nicotinamide adenine dinucleotide molecules: hydrolyzes NAD(H) into NMN(H) and AMP, and NADPH into NMNH and 2',5'-ADP (PubMed:12790796).
May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle (PubMed:12790796).
Regulates the levels of circadian clock components PER1, PER2, PER3 and CRY2 in the liver (By similarity).

Biological Process

Circadian regulation of gene expressionISS:UniProtKB
Methylguanosine-cap decappingManual Assertion Based On ExperimentIDA:UniProtKB
mRNA catabolic processISS:UniProtKB
NAD biosynthesis via nicotinamide riboside salvage pathwayTAS:Reactome
NAD catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
NAD-cap decappingManual Assertion Based On ExperimentIDA:UniProtKB
NADH metabolic processManual Assertion Based On ExperimentIBA:GO_Central
NADP catabolic processManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Peroxisome
Cytoplasmic granule
Localizes to cytoplasmic granules in the presence of BLMH.

Lukaszewicz, M., Ferenc-Mrozek, A., Bojarska, E., Stelmach, J., Stepinski, J., & Darzynkiewicz, E. (2023). Contribution of Nudt12 enzyme to differentially methylated dinucleotides of 5’RNA cap structure. Biochimica et Biophysica Acta (BBA)-General Subjects, 1867(9), 130400.

Wu, H., Li, L., Chen, K. M., Homolka, D., Gos, P., Fleury-Olela, F., ... & Pillai, R. S. (2019). Decapping enzyme NUDT12 partners with BLMH for cytoplasmic surveillance of NAD-capped RNAs. Cell reports, 29(13), 4422-4434.

Grudzien-Nogalska, E., Wu, Y., Jiao, X., Cui, H., Mateyak, M. K., Hart, R. P., ... & Kiledjian, M. (2019). Structural and mechanistic basis of mammalian Nudt12 RNA deNADding. Nature chemical biology, 15(6), 575-582.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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