Mouse Anti-P2RY4 Recombinant Antibody (CBT2877) (V2LY-0625-LY777)

Name: Mouse Anti-P2RY4 Recombinant Antibody (CBT2877)
SKU: V2LY-0625-LY777
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-P2RY4 Recombinant Antibody (CBT2877) (V2LY-0625-LY777) in ELISA

Mouse Anti-P2RY4 Recombinant Antibody (CBT2877) (V2LY-0625-LY777) in WB

Mouse Anti-P2RY4 Recombinant Antibody (CBT2877) (V2LY-0625-LY777) in IHC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2877

Application

WB, IHC

Immunogen

Purified recombinant fragment of human P2RY4 (AA: extra mix) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Pyrimidinergic Receptor P2Y4

Entrez Gene ID

5030

UniProt ID

P51582

Function

Receptor for UTP and UDP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Not activated by ATP or ADP.

Topology

Extracellular: 1-34
Helical: 35-61
Cytoplasmic: 62-72
Helical: 73-95
Extracellular: 96-112
Helical: 113-131
Cytoplasmic: 132-154
Helical: 155-174
Extracellular: 175-196
Helical: 197-222
Cytoplasmic: 223-246
Helical: 247-269
Extracellular: 270-287
Helical: 288-309
Cytoplasmic: 310-365

PTM

Phosphorylation of Ser-333 and Ser-334 is a key step in agonist-dependent desensitization and loss of surface P2RY4. This phosphorylation does not involve PKC, nor other calcium activated kinases.

Harata, A., Hirakawa, M., Sakuma, T., Yamamoto, T., & Hashimoto, C. (2019). Nucleotide receptor P2RY4 is required for head formation via induction and maintenance of head organizer in Xenopus laevis. Development, Growth & Differentiation, 61(2), 186-197.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-P2RY4 Recombinant Antibody (CBT3426) (CAT#: V2LY-0625-LY776)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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