Mouse Anti-PACSIN2 Recombinant Antibody (1735CT116.76.60) (CBMAB-P0632-YC)

Name: Mouse Anti-PACSIN2 Recombinant Antibody (1735CT116.76.60)
SKU: CBMAB-P0632-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1735CT116.76.60

Application

ELISA, WB

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, 0.09% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

protein kinase C and casein kinase substrate in neurons 2

Introduction

PACSIN2 is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation by regulating tubulin polymerization.

Entrez Gene ID

Human	11252
Mouse	23970

UniProt ID

Human	Q9UNF0
Mouse	Q9WVE8

Alternative Names

Protein Kinase C And Casein Kinase Substrate In Neurons 2; Syndapin-II; Syndapin-2; Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2; Cytoplasmic Phosphoprotein PACSIN2; Syndapin II; Syndapin2; SDPII;

Function

Regulates the morphogenesis and endocytosis of caveolae (By similarity).
Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus.
(Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer (PubMed:29891700).
Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes (PubMed:31242077).
Involved in lipid droplet formation, which is important for HCV virion assembly (PubMed:31801866).

Biological Process

Actin cytoskeleton organizationManual Assertion Based On ExperimentTAS:ProtInc
Caveola assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Caveolin-mediated endocytosisManual Assertion Based On ExperimentIMP:UniProtKB
Cell projection morphogenesisISS:UniProtKB
Cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Modulation of chemical synaptic transmissionIEA:Ensembl
Negative regulation of endocytosisIEA:Ensembl
Plasma membrane tubulationManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to endosomeManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of endocytosisManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm
Cytoplasm, cytoskeleton
Cytoplasmic vesicle membrane
Early endosome
Recycling endosome membrane
Cell projection, ruffle membrane
Cell membrane
Cell projection
Membrane, caveola
Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1.

PTM

Phosphorylated by casein kinase 2 (CK2). Phosphorylation by PKC probably decreases the membrane binding and tubulation capacities of PACSIN2, thereby modulating the lifetime of caveolae (By similarity).

Bouslama, R., Dumont, V., Lindfors, S., Paavolainen, L., Tienari, J., Nisen, H., ... & Lehtonen, S. (2023). Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP. Cells, 12(11), 1487.

Wint, H., Li, J., Abe, T., Yamada, H., Higaki, T., Nasu, Y., ... & Takeda, T. (2023). Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells. Journal of cell science, 136(10).

Biswas, R., Boyd, E. K., Eaton, N., Steenackers, A., Schulte, M. L., Reusswig, F., ... & Falet, H. (2023). PACSIN2 regulates platelet integrin β1 hemostatic function. Journal of Thrombosis and Haemostasis, 21(12), 3619-3632.

Morgan, J., Yarwood, R., Starborg, T., Yan, G., & Lowe, M. (2022). Pacsin2 is required for endocytosis in the zebrafish pronephric tubule. Biology Open, 11(6), bio059150.

Li, J., Fujise, K., Wint, H., Senju, Y., Suetsugu, S., Yamada, H., ... & Takeda, T. (2021). Dynamin 2 and BAR domain protein pacsin 2 cooperatively regulate formation and maturation of podosomes. Biochemical and Biophysical Research Communications, 571, 145-151.

Malinova, T. S., Angulo-Urarte, A., Nüchel, J., Tauber, M., van Der Stoel, M. M., Janssen, V., ... & Huveneers, S. (2021). A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis. Nature communications, 12(1), 2610.

Nguyen, L. P., Tran, S. C., Suetsugu, S., Lim, Y. S., & Hwang, S. B. (2020). PACSIN2 interacts with nonstructural protein 5A and regulates hepatitis C virus assembly. Journal of Virology, 94(5), 10-1128.

Franca, R., Stocco, G., Favretto, D., Giurici, N., Del Rizzo, I., Locatelli, F., ... & Rabusin, M. (2020). PACSIN2 rs2413739 influence on thiopurine pharmacokinetics: validation studies in pediatric patients. The Pharmacogenomics Journal, 20(3), 415-425.

Sanderlin, A. G., Vondrak, C., Scricco, A. J., Fedrigo, I., Ahyong, V., & Lamason, R. L. (2019). RNAi screen reveals a role for PACSIN2 and caveolins during bacterial cell-to-cell spread. Molecular biology of the cell, 30(17), 2124-2133.

Postema, M. M., Grega-Larson, N. E., Meenderink, L. M., & Tyska, M. J. (2019). PACSIN2-dependent apical endocytosis regulates the morphology of epithelial microvilli. Molecular biology of the cell, 30(19), 2515-2526.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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