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Mouse Anti-PADI4 Monoclonal Antibody (4D8) (CBMAB-1437-YC)

Provided herein is a mouse monoclonal antibody against Human PADI4. The antibody, clone 4D8, can be used for immunoassay techniques, such as ELISA, ICC, IF, IHC, IHC-P and WB.
See all PADI4 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
4D8
Antibody Isotype
IgG2b, κ
Application
ELISA, ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
Partial recombinant protein PADI4 (aa 2-111) with GST tag.
Specificity
Human, Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
peptidyl arginine deiminase, type IV
Introduction
PADI4 is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. PADI4 may play a role in granulocyte and macrophage development leading to inflammation and immune response.
Entrez Gene ID
Human23569
Mouse18602
UniProt ID
HumanQ9UM07
MouseQ9Z183
Alternative Names
PAD; PAD4; PDI4; PDI5; PADI5
Function
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532).
Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:18209087).
Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity).
Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed:15345777).
Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed:15731352).
Biological Process
Cellular protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Chromatin organizationISS:UniProtKB
Chromatin remodelingISS:UniProtKB
Histone citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Histone H3-R26 citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Innate immune responseIEA:UniProtKB-KW
Nucleosome assemblyISS:UniProtKB
Protein citrullinationManual Assertion Based On ExperimentIDA:UniProtKB
Stem cell population maintenanceISS:UniProtKB
Cellular Location
Cytoplasm
Nucleus
Cytoplasmic granule
Cytoplasmic granules of eosinophils and neutrophils.
Involvement in disease
Rheumatoid arthritis (RA):
An inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
PTM
Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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