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Mouse Anti-PAICS Recombinant Antibody (2C9) (CBMAB-P0680-YC)

Provided herein is a Mouse monoclonal antibody against Human Phosphoribosylaminoimidazole Carboxylase And Phosphoribosylaminoimidazolesuccinocarboxamide Synthase. The antibody can be used for immunoassay techniques, such as FC, ICC, IF, IHC, WB.
See all PAICS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C9
Antibody Isotype
IgG1
Application
FC, ICC, IF, IHC, WB

Basic Information

Immunogen
Full length human recombinant protein of human PAICS (NP_006443) produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
Introduction
PAICS is a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene.
Entrez Gene ID
10606
UniProt ID
P22234
Alternative Names
Phosphoribosylaminoimidazole Carboxylase And Phosphoribosylaminoimidazolesuccinocarboxamide Synthase; Phosphoribosylaminoimidazole Carboxylase, Phosphoribosylaminoimidazole Succinocarboxamide Synthetase; PAIS; ADE2; AIRC; Phosphoribosylaminoimidazole Carboxylase; Phosphoribosylaminoimidazolesuccinocarboxamide Synthase;
Function
Bifunctional phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazole succinocarboxamide synthetase catalyzing two reactions of the de novo purine biosynthetic pathway.
Biological Process
de novo' AMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
'de novo' IMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
'de novo' XMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
GMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Purine nucleobase biosynthetic processManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
cytoplasm
cytosol
extracellular exosome
membrane

Huo, A., & Xiong, X. (2023). PAICS as a potential target for cancer therapy linking purine biosynthesis to cancer progression. Life Sciences, 122070.

Prejanò, M., Skerlova, J., Stenmark, P., & Himo, F. (2022). Reaction mechanism of human PAICS elucidated by quantum chemical calculations. Journal of the American Chemical Society, 144(31), 14258-14268.

Lang, L., Tao, J., Yang, C., & Li, W. (2022). Tumor suppressive role of microRNA-4731-5p in breast cancer through reduction of PAICS-induced FAK phosphorylation. Cell Death Discovery, 8(1), 154.

He, J., Zou, L. N., Pareek, V., & Benkovic, S. J. (2022). Multienzyme interactions of the de novo purine biosynthetic protein PAICS facilitate purinosome formation and metabolic channeling. Journal of Biological Chemistry, 298(5).

Tang, X., Wu, Y., Yang, J., & Zhu, W. (2021). Regulating COX10-AS1/miR-142-5p/PAICS axis inhibits the proliferation of non-small cell lung cancer. Bioengineered, 12(1), 4643-4653.

Agarwal, S., Chakravarthi, B. V., Kim, H. G., Gupta, N., Hale, K., Balasubramanya, S. A. H., ... & Varambally, S. (2020). PAICS, a de novo purine biosynthetic enzyme, is overexpressed in pancreatic cancer and is involved in its progression. Translational Oncology, 13(7), 100776.

Huang, N., Xu, C., Deng, L., Li, X., Bian, Z., Zhang, Y., ... & Sun, F. (2020). PAICS contributes to gastric carcinogenesis and participates in DNA damage response by interacting with histone deacetylase 1/2. Cell Death & Disease, 11(7), 507.

Agarwal, S., Chakravarthi, B. V., Behring, M., Kim, H. G., Chandrashekar, D. S., Gupta, N., ... & Manne, U. (2020). PAICS, a purine nucleotide metabolic enzyme, is involved in tumor growth and the metastasis of colorectal cancer. Cancers, 12(4), 772.

Zhou, S., Yan, Y., Chen, X., Wang, X., Zeng, S., Qian, L., ... & Xu, Z. (2019). Roles of highly expressed PAICS in lung adenocarcinoma. Gene, 692, 1-8.

Pelet, A., Skopova, V., Steuerwald, U., Baresova, V., Zarhrate, M., Plaza, J. M., ... & Zikanova, M. (2019). PAICS deficiency, a new defect of de novo purine synthesis resulting in multiple congenital anomalies and fatal outcome. Human Molecular Genetics, 28(22), 3805-3814.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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