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Mouse Anti-PAICS Recombinant Antibody (4F4) (CBMAB-A6302-LY)

The product is antibody recognizes PAICS. The antibody 4F4 immunoassay techniques such as: WB, ELISA.
See all PAICS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
PAICS (AAH10273, 1 a.a. ~ 425 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
Introduction
This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
10606
UniProt ID
P22234
Alternative Names
ADE2; ADE2H1; AIRC; DKFZp781N1372; MGC1343; MGC5024; PAIS
Function
Bifunctional phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazole succinocarboxamide synthetase catalyzing two reactions of the de novo purine biosynthetic pathway.
Biological Process
de novo' AMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
'de novo' IMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
'de novo' XMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
GMP biosynthetic processManual Assertion Based On ExperimentIDA:MGI
Purine nucleobase biosynthetic processManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
cytoplasm
cytosol
extracellular exosome
membrane

Huo, A., & Xiong, X. (2023). PAICS as a potential target for cancer therapy linking purine biosynthesis to cancer progression. Life Sciences, 122070.

Prejanò, M., Skerlova, J., Stenmark, P., & Himo, F. (2022). Reaction mechanism of human PAICS elucidated by quantum chemical calculations. Journal of the American Chemical Society, 144(31), 14258-14268.

Lang, L., Tao, J., Yang, C., & Li, W. (2022). Tumor suppressive role of microRNA-4731-5p in breast cancer through reduction of PAICS-induced FAK phosphorylation. Cell Death Discovery, 8(1), 154.

He, J., Zou, L. N., Pareek, V., & Benkovic, S. J. (2022). Multienzyme interactions of the de novo purine biosynthetic protein PAICS facilitate purinosome formation and metabolic channeling. Journal of Biological Chemistry, 298(5).

Tang, X., Wu, Y., Yang, J., & Zhu, W. (2021). Regulating COX10-AS1/miR-142-5p/PAICS axis inhibits the proliferation of non-small cell lung cancer. Bioengineered, 12(1), 4643-4653.

Agarwal, S., Chakravarthi, B. V., Kim, H. G., Gupta, N., Hale, K., Balasubramanya, S. A. H., ... & Varambally, S. (2020). PAICS, a de novo purine biosynthetic enzyme, is overexpressed in pancreatic cancer and is involved in its progression. Translational Oncology, 13(7), 100776.

Huang, N., Xu, C., Deng, L., Li, X., Bian, Z., Zhang, Y., ... & Sun, F. (2020). PAICS contributes to gastric carcinogenesis and participates in DNA damage response by interacting with histone deacetylase 1/2. Cell Death & Disease, 11(7), 507.

Agarwal, S., Chakravarthi, B. V., Behring, M., Kim, H. G., Chandrashekar, D. S., Gupta, N., ... & Manne, U. (2020). PAICS, a purine nucleotide metabolic enzyme, is involved in tumor growth and the metastasis of colorectal cancer. Cancers, 12(4), 772.

Zhou, S., Yan, Y., Chen, X., Wang, X., Zeng, S., Qian, L., ... & Xu, Z. (2019). Roles of highly expressed PAICS in lung adenocarcinoma. Gene, 692, 1-8.

Pelet, A., Skopova, V., Steuerwald, U., Baresova, V., Zarhrate, M., Plaza, J. M., ... & Zikanova, M. (2019). PAICS deficiency, a new defect of de novo purine synthesis resulting in multiple congenital anomalies and fatal outcome. Human Molecular Genetics, 28(22), 3805-3814.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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