Mouse Anti-PAPD7 Recombinant Antibody (2F8) (CBMAB-P0743-YC)

Name: Mouse Anti-PAPD7 Recombinant Antibody (2F8)
SKU: CBMAB-P0743-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F8

Application

ELISA, RNAi, WB

Immunogen

POLS (NP_008930, 2-110 aa) partial recombinant protein with GST tag. Immunogen sequence: SPCPEEAAMR REVVKRIETV VKDLWPTADV QIFGSFSTGL YLPTSDIDLV VFGKWERPPL QLLEQALRKH NVAEPCSIKV LDKATVPIIK LTDQETEVKV DISFNMETG

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 2-110

More Infomation

Target

Full Name

Poly(A) RNA Polymerase D7, Non-Canonical

Introduction

PAPD7 is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described.

Entrez Gene ID

11044

UniProt ID

Q5XG87

Alternative Names

Poly(A) RNA Polymerase D7, Non-Canonical; Topoisomerase-Related Function Protein 4-1; TRAMP-Like Complex Polyadenylate Polymerase; PAP-Associated Domain-Containing Protein 7; PAP Associated Domain Containing 7; Polymerase (DNA Directed) Sigma; Terminal Uridylyltransferase 5; DNA Polymerase Kappa; DNA Polymerase Sigma; TUTase 5; TUTase5;

Function

Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:23376078, PubMed:30026317).
Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation (PubMed:23376078).

Biological Process

Double-strand break repair1 PublicationNAS:UniProtKB
Mitotic chromosome condensation1 PublicationNAS:UniProtKB
mRNA processingIEA:UniProtKB-KW
Negative regulation of nuclear-transcribed mRNA poly(A) tail shorteningManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of 3'-UTR-mediated mRNA stabilizationManual Assertion Based On ExperimentIMP:UniProtKB
Response to xenobiotic stimulusManual Assertion Based On ExperimentIDA:UniProtKB
RNA 3' uridylationManual Assertion Based On ExperimentIMP:UniProtKB
RNA 3'-end processingManual Assertion Based On ExperimentIBA:GO_Central
RNA polyadenylationIEA:InterPro
Sister chromatid cohesionManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cytoplasm
Nucleus, nucleoplasm
Excluded from nucleolus, weak staining detected in the cytoplasm.

Liu, F., Lee, A. C., Guo, F., Kondratowicz, A. S., Micolochick Steuer, H. M., Miller, A., ... & Gao, M. (2021). Host poly (A) polymerases PAPD5 and PAPD7 provide two layers of protection that ensure the integrity and stability of hepatitis B virus RNA. Journal of Virology, 95(18), 10-1128.

Sun, L., Zhang, F., Guo, F., Liu, F., Kulsuptrakul, J., Puschnik, A., ... & Zhou, T. (2020). The dihydroquinolizinone compound RG7834 inhibits the polyadenylase function of PAPD5 and PAPD7 and accelerates the degradation of matured hepatitis B virus surface protein mRNA. Antimicrobial Agents and Chemotherapy, 65(1), 10-1128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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