Mouse Anti-PBK Recombinant Antibody (3A7) (CBMAB-1656-CN)

Name: Mouse Anti-PBK Recombinant Antibody (3A7)
SKU: CBMAB-1656-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A7

Application

WB, ELISA, IF, IHC, IHC-P

Immunogen

PBK (AAH15191, aa. 1-110) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2b, Κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

PDZ Binding Kinase

Introduction

PBK is a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. PBK may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of PBK has been implicated in tumorigenesis.

Entrez Gene ID

55872

UniProt ID

Q96KB5

Alternative Names

SPK; CT84; TOPK; HEL164; Nori-3

Function

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

Biological Process

Cellular response to UVIEA:Ensembl
Mitotic cell cycle1 PublicationNAS:UniProtKB
Negative regulation of inflammatory responseIEA:Ensembl
Negative regulation of proteasomal ubiquitin-dependent protein catabolic processIEA:Ensembl
Negative regulation of protein phosphorylationIEA:Ensembl
Negative regulation of stress-activated MAPK cascadeIEA:Ensembl

Cellular Location

Nucleus

PTM

Phosphorylated; in a cell-cycle dependent manner at mitosis.

Wu, H., Qian, J., Zhou, L., Hu, T., Zhang, Y., Wang, C., ... & Gu, C. (2024). FHND004 inhibits malignant proliferation of multiple myeloma by targeting PDZ-binding kinase in MAPK pathway. Aging (Albany NY), 16(5), 4811.

Mu, W., Xie, Y., Li, J., Yan, R., Zhang, J., Liu, Y. E., & Fan, Y. (2022). High expression of PDZ-binding kinase is correlated with poor prognosis and immune infiltrates in hepatocellular carcinoma. World Journal of Surgical Oncology, 20(1), 22.

Miki, Y., Devi, L., Imai, Y., Minami, N., Koide, T., & Goel, S. (2020). Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice. Reproduction, Fertility and Development, 32(10), 893-902.

Mao, P., Bao, G., Wang, Y. C., Du, C. W., Yu, X., Guo, X. Y., ... & Wang, M. D. (2020). PDZ-binding kinase-dependent transcriptional regulation of CCNB2 promotes tumorigenesis and radio-resistance in glioblastoma. Translational oncology, 13(2), 287-294.

Chen, D., Cox, J., Annam, J., Weingart, M., Essien, G., Rathi, K. S., ... & Schnepp, R. W. (2020). LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase. Neoplasia, 22(6), 231-241.

Kar, A., Zhang, Y., Yacob, B. W., Saeed, J., Tompkins, K. D., Bagby, S. M., ... & Kiseljak-Vassiliades, K. (2019). Targeting PDZ-binding kinase is anti-tumorigenic in novel preclinical models of ACC. Endocrine-related cancer, 26(10), 765-778.

Warren, A. Y., Massie, C. E., Watt, K., Luko, K., Orafidiya, F., Selth, L. A., ... & Asim, M. (2019). A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer. Oncogene, 38(7), 1136-1150.

Zhang, Y., Yang, X., Wang, R., & Zhang, X. (2019). Prognostic value of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in patients with cancer. Journal of Cancer, 10(1), 131.

Diao, X., Yang, D., Chen, Y., & Liu, W. (2019). Baicalin suppresses lung cancer growth by targeting PDZ-binding kinase/T-LAK cell-originated protein kinase. Bioscience Reports, 39(4), BSR20181692.

Kruthika, B. S., Jain, R., Arivazhagan, A., Bharath, R. D., Yasha, T. C., Kondaiah, P., & Santosh, V. (2019). Transcriptome profiling reveals PDZ binding kinase as a novel biomarker in peritumoral brain zone of glioblastoma. Journal of neuro-oncology, 141, 315-325.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

PBK Matched Antibody Pair (845) (CAT#: APMAB-845LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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