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Mouse Anti-PDK1 Recombinant Antibody (4A11) (CBMAB-P1314-YC)

Provided herein is a Mouse monoclonal antibody against Human Pyruvate Dehydrogenase Kinase 1. The antibody can be used for immunoassay techniques, such as ELISA, FC, ICC, IF, IHC, IHC-P, WB.
See all PDK1 antibodies
Published Data
Fig.1 The effects of ox-LDL (0, 25, 50 or 100 ug/ml) on PDK1 expressions were detected via RT-qPCR and western blot.
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Fig.2 Immunocytochemical analysis of NB cells cultured in low oxygen concentration.
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Fig.3 Confocal microscopy confirmed cellular colocalization (yellow, arrows) of PDK1 (green) in MPs (Iba-1, red) at W4 of CPZ diet.
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Summary

Host Animal
Mouse
Specificity
Human, Monkey, Rat
Clone
4A11
Antibody Isotype
IgG1
Application
ELISA, FC, ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
Human PDK1 expressed in E. coli
Specificity
Human, Monkey, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
0.03% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Pyruvate Dehydrogenase Kinase 1
Introduction
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple
Entrez Gene ID
Human5163
Rat116551
Monkey697772
UniProt ID
HumanQ15118
RatO55173
MonkeyF7GNV0
Alternative Names
Pyruvate Dehydrogenase Kinase 1; EC 2.7.11.2; Pyruvate Dehydrogenase Kinase, Isoenzyme 1; Pyruvate Dehydrogenase Kinase, Isozyme 1; Pyruvate Dehydrogenase Kinase Isoform 1; PDH Kinase 1; EC 2.7.11; PDHK1;
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Biological Process
Cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Glucose metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Hypoxia-inducible factor-1alpha signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stressManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of acetyl-CoA biosynthetic process from pyruvateTAS:Reactome
Regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Mitochondrion matrix
PTM
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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