Mouse Anti-PDK1 Recombinant Antibody (4A11) (CBMAB-P1314-YC)

Name: Mouse Anti-PDK1 Recombinant Antibody (4A11)
SKU: CBMAB-P1314-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

4A11

Application

ELISA, FC, ICC, IF, IHC, IHC-P, WB

Immunogen

Human PDK1 expressed in E. coli

Specificity

Human, Monkey, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

0.03% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Pyruvate Dehydrogenase Kinase 1

Introduction

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple

Entrez Gene ID

Human	5163
Rat	116551
Monkey	697772

UniProt ID

Human	Q15118
Rat	O55173
Monkey	F7GNV0

Alternative Names

Pyruvate Dehydrogenase Kinase 1; EC 2.7.11.2; Pyruvate Dehydrogenase Kinase, Isoenzyme 1; Pyruvate Dehydrogenase Kinase, Isozyme 1; Pyruvate Dehydrogenase Kinase Isoform 1; PDH Kinase 1; EC 2.7.11; PDHK1;

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Biological Process

Cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Glucose metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Hypoxia-inducible factor-1alpha signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stressManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of acetyl-CoA biosynthetic process from pyruvateTAS:Reactome
Regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Mitochondrion matrix

PTM

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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