Mouse Anti-PDK1 Recombinant Antibody (CBT1013) (V2LY-0625-LY3109)

Name: Mouse Anti-PDK1 Recombinant Antibody (CBT1013)
SKU: V2LY-0625-LY3109
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-PDK1 Recombinant Antibody (CBT1013) (V2LY-0625-LY3109) in FC

Mouse Anti-PDK1 Recombinant Antibody (CBT1013) (V2LY-0625-LY3109) in IF

Mouse Anti-PDK1 Recombinant Antibody (CBT1013) (V2LY-0625-LY3109) in IHC

Mouse Anti-PDK1 Recombinant Antibody (CBT1013) (V2LY-0625-LY3109) in WB

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT1013

Application

WB, IHC-P, IF, ICC, FC, ELISA

Immunogen

Purified recombinant fragment of human PDK1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
FC	1:200-1:400
IF	1:200-1:1,000
IHC	1:200-1:1,000
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

BSA, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Pyruvate Dehydrogenase Kinase 1

Entrez Gene ID

5163

UniProt ID

Q15118

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Biological Process

Cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Glucose metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Hypoxia-inducible factor-1alpha signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Intrinsic apoptotic signaling pathway in response to oxidative stressManual Assertion Based On ExperimentIMP:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of acetyl-CoA biosynthetic process from pyruvateTAS:Reactome
Regulation of glucose metabolic processManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Mitochondrion matrix

PTM

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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