Rabbit Anti-PGF Recombinant Antibody (
1B6) (V2LY-0725-LY1157)

Name: Rabbit Anti-PGF Recombinant Antibody (1B6)
SKU: V2LY-0725-LY1157
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-PGF Recombinant Antibody (1B6) (V2LY-0725-LY1157) in IHC

IHC image of V2LY-0725-LY1157 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-PGF Recombinant Antibody (1B6) (V2LY-0725-LY1157) in IHC

IHC image of V2LY-0725-LY1157 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

1B6

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human PLGF.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Placental Growth Factor

Entrez Gene ID

5228

UniProt ID

P49763

Function

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Biological Process

Animal organ regenerationIEA:Ensembl
Cell differentiationIEA:UniProtKB-KW
Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to hormone stimulusIEA:Ensembl
Female pregnancyIEA:Ensembl
Induction of positive chemotaxisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of angiogenesisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell divisionIEA:UniProtKB-KW
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of endothelial cell proliferationManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of mast cell chemotaxisManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of protein phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Response to hypoxiaManual Assertion Based On ExperimentIBA:GO_Central
Response to xenobiotic stimulusIEA:Ensembl
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Sprouting angiogenesisManual Assertion Based On ExperimentIBA:GO_Central
Vascular endothelial growth factor receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Vascular endothelial growth factor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Secreted
The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

PTM

N-glycosylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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