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Mouse Anti-PHIP Recombinant Antibody (4D7) (CBMAB-P1678-YC)

Provided herein is a Mouse monoclonal antibody against Human Pleckstrin Homology Domain Interacting Protein. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all PHIP antibodies
Published Data
Fig. 1 Western blot analysis of Phip and Gapdh in B16-F10 stable transformants expressing anti-luc shRNA (lane 1) or anti-Phip shRNA (lane 2). Phip levels were reduced by 70% in anti-Phip shRNA-expressing cells.
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Fig. 2 Immunofluorescent detection of PHIP
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4D7
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
PHIP (NP_060404, 1599-1705 aa) partial recombinant protein with GST tag. Immunogen sequence: TLSKSSAVIE QGDCKNNALV PGTIQVNGHG GQPSKLVKRG PGRKPKVEVN TNSGEIIHKK RGRKPKKLQY AKPEDLEQNN VHPIRDEVLP SSTCNFLSET NNVKEDL
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1599-1705

Target

Full Name
pleckstrin homology domain interacting protein
Introduction
PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. The encoded protein may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.
Entrez Gene ID
55023
UniProt ID
Q8WWQ0
Alternative Names
Pleckstrin Homology Domain Interacting Protein; DDB1 And CUL4 Associated Factor 14; IRS-1 PH Domain-Binding Protein; WD Repeat-Containing Protein 11; WDR11; PH-Interacting Protein; DCAF14; BRWD2; Ndrp;
Function
Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti-apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization.
Biological Process
Cytoskeleton organizationManual Assertion Based On ExperimentIMP:UniProtKB
Insulin receptor signaling pathway1 PublicationNAS:UniProtKB
Negative regulation of apoptotic processISS:UniProtKB
Negative regulation of extrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell population proliferationISS:UniProtKB
Positive regulation of insulin-like growth factor receptor signaling pathwayISS:UniProtKB
Positive regulation of mitotic nuclear divisionISS:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:MGI
Positive regulation of transcription, DNA-templatedISS:UniProtKB
Regulation of cell morphogenesisManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of cell shapeManual Assertion Based On ExperimentIBA:GO_Central
Regulation of protein phosphorylationISS:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Involvement in disease
Chung-Jansen syndrome (CHUJANS):
An autosomal dominant disorder characterized by developmental delay, intellectual disability, autistic features, anxiety, hypotonia, obesity, and dysmorphic features.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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