Mouse Anti-PKMYT1 Recombinant Antibody (1C3) (CBMAB-P1944-YC)

Name: Mouse Anti-PKMYT1 Recombinant Antibody (1C3)
SKU: CBMAB-P1944-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1C3

Application

ICC, IF, WB

Immunogen

Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

protein kinase, membrane associated tyrosine/threonine 1

Introduction

PKMYT1 is a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1.

Entrez Gene ID

9088

UniProt ID

Q99640

Alternative Names

PPP1R126; MYT1

Function

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Biological Process

G2/M transition of mitotic cell cycleTAS:Reactome
Meiotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Regulation of cyclin-dependent protein serine/threonine kinase activityManual Assertion Based On ExperimentTAS:ProtInc
Regulation of mitotic nuclear divisionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Endoplasmic reticulum membrane
Golgi apparatus membrane

PTM

Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-PKMYT1 Recombinant Antibody (2B4) (CAT#: CBMAB-P1946-YC)

Mouse Anti-PKMYT1 Recombinant Antibody (50) (CAT#: CBMAB-P1947-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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