Mouse Anti-PLIN3 Recombinant Antibody (CBT2378) (V2LY-0625-LY1925)

Name: Mouse Anti-PLIN3 Recombinant Antibody (CBT2378)
SKU: V2LY-0625-LY1925
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-PLIN3 Recombinant Antibody (CBT2378) (V2LY-0625-LY1925) in WB

Mouse Anti-PLIN3 Recombinant Antibody (CBT2378) (V2LY-0625-LY1925) in IF

Mouse Anti-PLIN3 Recombinant Antibody (CBT2378) (V2LY-0625-LY1925) in FC

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT2378

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human PLIN3 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:500
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

mannose-6-phosphate receptor binding protein 1

Entrez Gene ID

10226

UniProt ID

O60664

Function

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets (PubMed:34077757).
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network (PubMed:9590177).

Biological Process

Cellular response to glucose starvationManual Assertion Based On ExperimentIDA:UniProtKB
Lipid droplet disassemblyManual Assertion Based On ExperimentIDA:UniProtKB
Lipid storageManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of sequestering of triglycerideManual Assertion Based On ExperimentIBA:GO_Central
Vesicle-mediated transportManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Lipid droplet
Endosome membrane
Cytoplasm
Membrane associated on endosomes (PubMed:15545278).
Detected in the envelope and the core of lipid bodies and in lipid sails (PubMed:15545278).

PTM

Phosphorylation at Tyr-251 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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