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Mouse Anti-POU2F1 Recombinant Antibody (POU5I097) (CBMAB-1796-YC)

Provided herein is a Mouse monoclonal antibody against Human POU2F1. The antibody, clone POU5I097, can be used for immunoassay techniques, such as WB, FC, Dot, ICC, IF.
See all POU2F1 antibodies
Published Data
Fig.1 Cos7 cells plated in 60 mm cell culture plates were transfected with 700 ng pGL3-LTR-luc, 105 ng phRL-TK, 315 ng pSG5 in combination with 280 ng Oct1, HNF3, c-Jun, or c-Fos expression plasmids.
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Fig.2 Immunohistochemical detection of Oct-1 expression in HCAECs 48 h after liposomal-based transfection of Oct-1 (50 nM).
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Summary

Host Animal
Mouse
Specificity
Human
Clone
POU5I097
Antibody Isotype
IgG1
Application
WB, FC, Dot, ICC, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
POU class 2 homeobox 1
Introduction
POU2F1 was among the first identified members of the POU transcription factor family. POU2F1 contains the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.
Entrez Gene ID
5451
UniProt ID
P14859
Alternative Names
3-Phosphoglyceric phosphokinase
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
(Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Biological Process
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Phosphorylated by PRKDC.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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