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Mouse Anti-PRC1 Recombinant Antibody (16F2) (CBMAB-P2684-YC)

Provided herein is a Mouse monoclonal antibody against Human Protein Regulator Of Cytokinesis 1. The antibody can be used for immunoassay techniques, such as WB, ICC, IF.
See all PRC1 antibodies
Published Data
Fig.3 Cells expressing dsRed-centrin were stained for IFT20 and PRC.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
16F2
Antibody Isotype
IgG2b
Application
WB, ICC, IF

Basic Information

Immunogen
Recombinant PRC1 protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
protein regulator of cytokinesis 1
Introduction
PRC1 is a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis.
Entrez Gene ID
Human9055
Mouse233406
Rat308761
UniProt ID
HumanO43663
MouseQ99K43
RatD3ZES9
Alternative Names
ASE1
Function
Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
Biological Process
Cell divisionIEA:UniProtKB-KW
Microtubule bundle formationIEA:InterPro
Microtubule cytoskeleton organizationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic spindle elongationManual Assertion Based On ExperimentTAS:ProtInc
Mitotic spindle midzone assemblyManual Assertion Based On ExperimentIBA:GO_Central
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of cytokinesisManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Cytoplasm
Cytoplasm, cytoskeleton, spindle pole
Midbody
Chromosome
Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436).
Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. Co-localizes with PRC1 in early mitosis and at the spindle midzone from anaphase B to telophase (PubMed:15297875, PubMed:15625105).
PTM
Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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