Mouse Anti-PRDM5 Recombinant Antibody (CBT2638) (V2LY-0625-LY398)

Name: Mouse Anti-PRDM5 Recombinant Antibody (CBT2638)
SKU: V2LY-0625-LY398
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-PRDM5 Recombinant Antibody (CBT2638) (V2LY-0625-LY398) in ELISA

Mouse Anti-PRDM5 Recombinant Antibody (CBT2638) (V2LY-0625-LY398) in WB

Mouse Anti-PRDM5 Recombinant Antibody (CBT2638) (V2LY-0625-LY398) in IF

Mouse Anti-PRDM5 Recombinant Antibody (CBT2638) (V2LY-0625-LY398) in FC

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2638

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:500
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

PRDM5

Entrez Gene ID

11107

UniProt ID

Q9NQX1

Function

Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May cause G2/M arrest and apoptosis in cancer cells.

Biological Process

Cellular response to leukemia inhibitory factorIEA:Ensembl
Chromatin organizationIEA:UniProtKB-KW
Histone deacetylationManual Assertion Based On ExperimentIMP:UniProtKB
Histone H3-K9 methylationManual Assertion Based On ExperimentIDA:UniProtKB
Mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:NTNU_SB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:ARUK-UCL
Regulation of extracellular matrix organizationManual Assertion Based On ExperimentIMP:ARUK-UCL
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus

Involvement in disease

Brittle cornea syndrome 2 (BCS2):
A disorder characterized by extreme corneal thinning resulting in corneal rupture after minor trauma, blue sclerae, keratoconus or keratoglobus, hyperelasticity of the skin, and hypermobile joints.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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