Mouse Anti-PRDX3 Recombinant Antibody (
12A10C12) (V2LY-0725-LY174)

Name: Mouse Anti-PRDX3 Recombinant Antibody (12A10C12)
SKU: V2LY-0725-LY174
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in WB

Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: PRDX3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 KDa Exposure time:5min

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in IHC

IHC image of V2LY-0725-LY174 diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in IHC

IHC image of V2LY-0725-LY174 diluted at 1:50 and staining in paraffin-embedded human endometrial cance tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in IF

Immunofluorescence staining of Hela cells with(V2LY-0725-LY174)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in IF

Immunofluorescence staining of MCF7 cells with(V2LY-0725-LY174)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Mouse Anti-PRDX3 Recombinant Antibody (12A10C12) (V2LY-0725-LY174) in FC

Overlay Peak curve showing Hela cells stained with V2LY-0725-LY174 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

12A10C12

Application

ELISA, WB, IHC, IF, FC

Immunogen

Recombinant Human Thioredoxin-dependent peroxide reductase, mitochondrial protein (63-256AA).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:5,000
WB	1:1,000-1:5,000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

PRDX3

Entrez Gene ID

10935

UniProt ID

P30048

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:7733872, PubMed:17707404).
Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477).

Biological Process

Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to oxidative stressManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:UniProtKB
Hydrogen peroxide catabolic processManual Assertion Based On ExperimentIDA:MGI
Maternal placenta developmentIEA:Ensembl
Mitochondrion organizationManual Assertion Based On ExperimentIMP:UniProtKB
Myeloid cell differentiationISS:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Negative regulation of kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-cysteine oxidationManual Assertion Based On ExperimentIDA:ParkinsonsUK-UCL
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of mitochondrial membrane potentialManual Assertion Based On ExperimentIMP:UniProtKB
Response to hydrogen peroxideManual Assertion Based On ExperimentIDA:UniProtKB
Response to lipopolysaccharideISS:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Mitochondrion
Cytoplasm
Early endosome
Localizes to early endosomes in a RPS6KC1-dependent manner.

PTM

Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.
S-palmitoylated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

PRDX3 Matched Antibody Pair (924) (CAT#: APMAB-924LY)

PRDX3 Matched Antibody Pair (925) (CAT#: APMAB-925LY)

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PRDX3 Matched Antibody Pair (924) (CAT#: APMAB-924LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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